Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12242 | 3' | -46.5 | NC_003309.1 | + | 52102 | 0.84 | 0.290552 |
Target: 5'- -uCCCGgugcgGAAAUGAAAAAGcCCCGCACGg -3' miRNA: 3'- guGGGU-----UUUUACUUUUUC-GGGCGUGC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 46671 | 0.7 | 0.937291 |
Target: 5'- gCGCUCAG--GUGAccGGGCCgGCGCGu -3' miRNA: 3'- -GUGGGUUuuUACUuuUUCGGgCGUGC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 44816 | 0.71 | 0.89048 |
Target: 5'- aAUUCGucAGUGAAGucGGCCCGCACu -3' miRNA: 3'- gUGGGUuuUUACUUUu-UCGGGCGUGc -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 39723 | 0.72 | 0.874322 |
Target: 5'- gCACCCAcu-AUGAGAAAGgCUGCAa- -3' miRNA: 3'- -GUGGGUuuuUACUUUUUCgGGCGUgc -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 37574 | 0.74 | 0.777815 |
Target: 5'- gACCgCGgcgucaacgucGAAGUGAAGGGGCCCGgGCGc -3' miRNA: 3'- gUGG-GU-----------UUUUACUUUUUCGGGCgUGC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 35457 | 0.66 | 0.993892 |
Target: 5'- cCGCCCGAugguGAUGGuuGGGuCCUGCuuGa -3' miRNA: 3'- -GUGGGUUu---UUACUuuUUC-GGGCGugC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 35425 | 0.72 | 0.874322 |
Target: 5'- gACCUAcGAcaccGAAAcGGCCCGCACGc -3' miRNA: 3'- gUGGGUuUUua--CUUUuUCGGGCGUGC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 27350 | 0.67 | 0.978033 |
Target: 5'- cCGCCCAAAAcgGGAGcGGCgCG-GCGa -3' miRNA: 3'- -GUGGGUUUUuaCUUUuUCGgGCgUGC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 27036 | 0.68 | 0.968804 |
Target: 5'- gACCCGAcucaGgcAAuacGCCCGCACGg -3' miRNA: 3'- gUGGGUUuuuaCuuUUu--CGGGCGUGC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 24775 | 0.68 | 0.968804 |
Target: 5'- gCACCCGAuuuUGGAugcAAAGCcCCGguCGa -3' miRNA: 3'- -GUGGGUUuuuACUU---UUUCG-GGCguGC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 21570 | 0.66 | 0.993892 |
Target: 5'- gCAUCCAcuccucgcaGAAAUGAAAAAG-CCGCccaaagGCGg -3' miRNA: 3'- -GUGGGU---------UUUUACUUUUUCgGGCG------UGC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 20635 | 0.71 | 0.882549 |
Target: 5'- uGCCCAGAAcgGuuc---CCCGCGCGa -3' miRNA: 3'- gUGGGUUUUuaCuuuuucGGGCGUGC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 20307 | 0.66 | 0.989949 |
Target: 5'- aCugCCGAucaucgcguucGUGAuccAGCCCGCACc -3' miRNA: 3'- -GugGGUUuu---------UACUuuuUCGGGCGUGc -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 16899 | 0.73 | 0.829038 |
Target: 5'- gCGCCgAGAAucGUGuuGAAGAGCCCGcCGCGc -3' miRNA: 3'- -GUGGgUUUU--UAC--UUUUUCGGGC-GUGC- -5' |
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12242 | 3' | -46.5 | NC_003309.1 | + | 8082 | 0.9 | 0.130338 |
Target: 5'- uGCCCcucacAAAUGAAAAGGCCCGCACGc -3' miRNA: 3'- gUGGGuu---UUUACUUUUUCGGGCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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