miRNA display CGI


Results 1 - 3 of 3 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12242 5' -45.9 NC_003309.1 + 46467 0.71 0.91872
Target:  5'- -gCGCGGUUGAGGUGCUCGAa----- -3'
miRNA:   3'- gaGCGCUAGUUCUACGGGUUaaauaa -5'
12242 5' -45.9 NC_003309.1 + 43617 0.66 0.993118
Target:  5'- uCUCGCGGugcuugUCcGGGUGCCCGu------ -3'
miRNA:   3'- -GAGCGCU------AGuUCUACGGGUuaaauaa -5'
12242 5' -45.9 NC_003309.1 + 13965 0.66 0.99508
Target:  5'- -aCGCGggCGGGA-GCCCGAUc---- -3'
miRNA:   3'- gaGCGCuaGUUCUaCGGGUUAaauaa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.