miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12243 3' -57.3 NC_003309.1 + 25391 0.66 0.605991
Target:  5'- -cUCGUGCCacUGCGuguaGCCGUC-GGCGUc -3'
miRNA:   3'- gcAGCACGG--ACGC----CGGCAGuUCGUAc -5'
12243 3' -57.3 NC_003309.1 + 48089 0.67 0.541348
Target:  5'- cCGUCGUGCUcgcacuucguguUGCGGUacuUCGAGCGc- -3'
miRNA:   3'- -GCAGCACGG------------ACGCCGgc-AGUUCGUac -5'
12243 3' -57.3 NC_003309.1 + 34524 0.67 0.540287
Target:  5'- cCGUCGaUGUggGCGGCCGUCGAaucgauuGCGc- -3'
miRNA:   3'- -GCAGC-ACGgaCGCCGGCAGUU-------CGUac -5'
12243 3' -57.3 NC_003309.1 + 39971 0.7 0.358225
Target:  5'- cCGUC-UGCCUcGCGGCgcgcuUGUCGAGCAc- -3'
miRNA:   3'- -GCAGcACGGA-CGCCG-----GCAGUUCGUac -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.