Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12244 | 3' | -57.5 | NC_003309.1 | + | 1988 | 0.66 | 0.585459 |
Target: 5'- --uGCGAcagguUCGUUGCGCCGuGCgGGuCg -3' miRNA: 3'- aagUGCU-----AGCAGCGCGGCuCGgUCuG- -5' |
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12244 | 3' | -57.5 | NC_003309.1 | + | 10637 | 0.66 | 0.574608 |
Target: 5'- -cCGCGAUgCG-CGCGCCGAuuuccugacuGCCGG-Ca -3' miRNA: 3'- aaGUGCUA-GCaGCGCGGCU----------CGGUCuG- -5' |
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12244 | 3' | -57.5 | NC_003309.1 | + | 43186 | 0.66 | 0.574608 |
Target: 5'- gUCACGucCGUCGCGCgaaccgcgucagCGAGCCcGAg -3' miRNA: 3'- aAGUGCuaGCAGCGCG------------GCUCGGuCUg -5' |
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12244 | 3' | -57.5 | NC_003309.1 | + | 16841 | 0.67 | 0.521207 |
Target: 5'- --uGCGAaucUCGUCGCGCCccaCCGGAUg -3' miRNA: 3'- aagUGCU---AGCAGCGCGGcucGGUCUG- -5' |
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12244 | 3' | -57.5 | NC_003309.1 | + | 568 | 0.67 | 0.520157 |
Target: 5'- cUUCGCGAaaggguugUCGgguguuuucggcuUUGCGCCGA-CCAGGCg -3' miRNA: 3'- -AAGUGCU--------AGC-------------AGCGCGGCUcGGUCUG- -5' |
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12244 | 3' | -57.5 | NC_003309.1 | + | 16758 | 0.67 | 0.510747 |
Target: 5'- -cCGCcGUCG-CGCGCCGAcGUCAGGa -3' miRNA: 3'- aaGUGcUAGCaGCGCGGCU-CGGUCUg -5' |
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12244 | 3' | -57.5 | NC_003309.1 | + | 44450 | 0.67 | 0.490099 |
Target: 5'- -aCACGA-CGUCGCccgGCCGAcuGCCGG-Cg -3' miRNA: 3'- aaGUGCUaGCAGCG---CGGCU--CGGUCuG- -5' |
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12244 | 3' | -57.5 | NC_003309.1 | + | 49955 | 0.67 | 0.479921 |
Target: 5'- aUUCACcGUCGauUCgGCgGCCGAGCCGGuCa -3' miRNA: 3'- -AAGUGcUAGC--AG-CG-CGGCUCGGUCuG- -5' |
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12244 | 3' | -57.5 | NC_003309.1 | + | 8262 | 0.69 | 0.411835 |
Target: 5'- cUCGCGAaugUCGUCGCGUCGAuuuCCuccGACu -3' miRNA: 3'- aAGUGCU---AGCAGCGCGGCUc--GGu--CUG- -5' |
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12244 | 3' | -57.5 | NC_003309.1 | + | 19783 | 0.7 | 0.333661 |
Target: 5'- -cCGCGuaaAUCGUCGCGUCGccGGCCAucGGCg -3' miRNA: 3'- aaGUGC---UAGCAGCGCGGC--UCGGU--CUG- -5' |
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12244 | 3' | -57.5 | NC_003309.1 | + | 13801 | 0.71 | 0.310135 |
Target: 5'- -aCGCGGUUGUUGuCGCCGccgcuuccGCCGGGCg -3' miRNA: 3'- aaGUGCUAGCAGC-GCGGCu-------CGGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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