miRNA: 3'- -UGCCGGUGa---GUGCG----AGU-------------AGCGCGUac -5'
miRNA: 3'- -UGC-CGGU-GAGUGCGAGUag---CG-CGUac -5'
Results 21 - 22 of 22 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12244 | 5' | -57 | NC_003309.1 | + | 47869 | 0.69 | 0.433893 |
|
Target: 5'- aGCGGCCAUgaagCGCGCuucgUCAcgucgccuccugaUCGCGCGg- -3' miRNA: 3'- -UGCCGGUGa---GUGCG----AGU-------------AGCGCGUac -5' |
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| 12244 | 5' | -57 | NC_003309.1 | + | 47964 | 0.67 | 0.536587 |
|
Target: 5'- gGCGcGCCAgCUCGCGCUCGgcgaaGCuGCGa- -3' miRNA: 3'- -UGC-CGGU-GAGUGCGAGUag---CG-CGUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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