Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12244 | 5' | -57 | NC_003309.1 | + | 43968 | 0.75 | 0.186871 |
Target: 5'- aGCGGUCGCgCACGCUC-UCGgGCAUc -3' miRNA: 3'- -UGCCGGUGaGUGCGAGuAGCgCGUAc -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 9185 | 0.68 | 0.46427 |
Target: 5'- cGCcGCCGC-CGCGaUCAUCGcCGCAUGc -3' miRNA: 3'- -UGcCGGUGaGUGCgAGUAGC-GCGUAC- -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 10888 | 0.68 | 0.453362 |
Target: 5'- cGCGGUCGCcgcgcguUC-CGCUCGcUCGCGCGc- -3' miRNA: 3'- -UGCCGGUG-------AGuGCGAGU-AGCGCGUac -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 16125 | 0.69 | 0.434855 |
Target: 5'- cGCGauGCCGCU-GCGCUCGcacaUCGCGCGa- -3' miRNA: 3'- -UGC--CGGUGAgUGCGAGU----AGCGCGUac -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 47869 | 0.69 | 0.433893 |
Target: 5'- aGCGGCCAUgaagCGCGCuucgUCAcgucgccuccugaUCGCGCGg- -3' miRNA: 3'- -UGCCGGUGa---GUGCG----AGU-------------AGCGCGUac -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 4688 | 0.7 | 0.379424 |
Target: 5'- cGCGGCCGCgcgagccgaaACGCUaCggCGCGCAUu -3' miRNA: 3'- -UGCCGGUGag--------UGCGA-GuaGCGCGUAc -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 46305 | 0.7 | 0.370657 |
Target: 5'- cACGGCCuucuGCgCACGCgag-CGCGCGUGc -3' miRNA: 3'- -UGCCGG----UGaGUGCGaguaGCGCGUAC- -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 43523 | 0.71 | 0.336992 |
Target: 5'- gGCGGCCAUcgCGCGCaUCAggUCGCgGCAg- -3' miRNA: 3'- -UGCCGGUGa-GUGCG-AGU--AGCG-CGUac -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 12650 | 0.71 | 0.328931 |
Target: 5'- uGCGaGCCGCUgcucgagauagcCGCGCUCG-CGCGCGa- -3' miRNA: 3'- -UGC-CGGUGA------------GUGCGAGUaGCGCGUac -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 10678 | 0.68 | 0.484447 |
Target: 5'- gGCGGCCGuCUCGCGggCGaugaUCGcCGCGUa -3' miRNA: 3'- -UGCCGGU-GAGUGCgaGU----AGC-GCGUAc -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 31715 | 0.67 | 0.525987 |
Target: 5'- gUGGCCGCaugUCugGCUCggCGCGaCGa- -3' miRNA: 3'- uGCCGGUG---AGugCGAGuaGCGC-GUac -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 11547 | 0.67 | 0.525987 |
Target: 5'- uGCGGCC----ACGUUCAUCGC-CGUGa -3' miRNA: 3'- -UGCCGGugagUGCGAGUAGCGcGUAC- -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 24590 | 0.66 | 0.579648 |
Target: 5'- uGCGGCUAaUC-UGCUCGUCuuGCGCAg- -3' miRNA: 3'- -UGCCGGUgAGuGCGAGUAG--CGCGUac -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 39849 | 0.66 | 0.568799 |
Target: 5'- uUGGCCcugcugaucCUCugGCUCGUCGUcgagguuacGCGUGc -3' miRNA: 3'- uGCCGGu--------GAGugCGAGUAGCG---------CGUAC- -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 43460 | 0.66 | 0.568799 |
Target: 5'- uCGGCUACUCA-GC-CAUgcCGCGCAg- -3' miRNA: 3'- uGCCGGUGAGUgCGaGUA--GCGCGUac -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 10485 | 0.67 | 0.558 |
Target: 5'- -aGGCU-UUCGCGCaUCG-CGCGCAUGc -3' miRNA: 3'- ugCCGGuGAGUGCG-AGUaGCGCGUAC- -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 22749 | 0.67 | 0.556923 |
Target: 5'- -gGGCCGucaguuugucgauCUCGCGCUgAUCGuCGCcgGc -3' miRNA: 3'- ugCCGGU-------------GAGUGCGAgUAGC-GCGuaC- -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 27207 | 0.67 | 0.547261 |
Target: 5'- aGCGGCgcaGC-CACGCUgccguugauUCGCGCGUGu -3' miRNA: 3'- -UGCCGg--UGaGUGCGAgu-------AGCGCGUAC- -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 47964 | 0.67 | 0.536587 |
Target: 5'- gGCGcGCCAgCUCGCGCUCGgcgaaGCuGCGa- -3' miRNA: 3'- -UGC-CGGU-GAGUGCGAGUag---CG-CGUac -5' |
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12244 | 5' | -57 | NC_003309.1 | + | 10845 | 0.67 | 0.536587 |
Target: 5'- -aGcGCgACaUCACGCUCGgcUCGCGCGa- -3' miRNA: 3'- ugC-CGgUG-AGUGCGAGU--AGCGCGUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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