miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12247 3' -57.6 NC_003309.1 + 45716 0.66 0.591965
Target:  5'- -gGGUCcgUGACGAGCgucaGCuuGUCAg -3'
miRNA:   3'- caCCAGuaGCUGCUCGac--UGggCGGU- -5'
12247 3' -57.6 NC_003309.1 + 14434 0.66 0.570393
Target:  5'- -aGGUauuugcCGUCGAccgucgcccCGAGCUGGCCaGCCGu -3'
miRNA:   3'- caCCA------GUAGCU---------GCUCGACUGGgCGGU- -5'
12247 3' -57.6 NC_003309.1 + 9421 0.67 0.5279
Target:  5'- -cGGcCGUCGACGAGa-GACCgCGCa- -3'
miRNA:   3'- caCCaGUAGCUGCUCgaCUGG-GCGgu -5'
12247 3' -57.6 NC_003309.1 + 33837 0.68 0.456718
Target:  5'- cGUGGcauUCGugaUCGACGuGUUcGCCCGCCGu -3'
miRNA:   3'- -CACC---AGU---AGCUGCuCGAcUGGGCGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.