Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12248 | 5' | -53.6 | NC_003309.1 | + | 10658 | 0.66 | 0.796672 |
Target: 5'- uGAUCGccgCGuACGGCaUGCCCGCGa- -3' miRNA: 3'- gUUAGCuaaGC-UGCCG-GCGGGUGUag -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 8470 | 0.68 | 0.680564 |
Target: 5'- -cAUUGA--CGAC-GCCGCCCACGUg -3' miRNA: 3'- guUAGCUaaGCUGcCGGCGGGUGUAg -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 7854 | 0.66 | 0.776694 |
Target: 5'- gCAGUCGAUcUGAuaggaaccggaaCGGCCGCCa--GUCa -3' miRNA: 3'- -GUUAGCUAaGCU------------GCCGGCGGgugUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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