Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12248 | 5' | -53.6 | NC_003309.1 | + | 49949 | 0.75 | 0.287789 |
Target: 5'- -cGUCGAUUCGGCGGCCGagCCG-GUCa -3' miRNA: 3'- guUAGCUAAGCUGCCGGCg-GGUgUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 29528 | 0.75 | 0.310436 |
Target: 5'- --uUCGGcagaUCGACGGCCGUCCGacCAUCg -3' miRNA: 3'- guuAGCUa---AGCUGCCGGCGGGU--GUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 23743 | 0.74 | 0.351138 |
Target: 5'- cCAAUCGAUcagaacagccCGACuGGCUGCgCCGCAUCg -3' miRNA: 3'- -GUUAGCUAa---------GCUG-CCGGCG-GGUGUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 27754 | 0.73 | 0.377319 |
Target: 5'- gAGUCGccagaCGACGcGCCGCCCggcGCAUCg -3' miRNA: 3'- gUUAGCuaa--GCUGC-CGGCGGG---UGUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 26853 | 0.73 | 0.404782 |
Target: 5'- aAGUCGAUcCGG-GGUCGCCgACAUCg -3' miRNA: 3'- gUUAGCUAaGCUgCCGGCGGgUGUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 52474 | 0.72 | 0.423777 |
Target: 5'- gCAAgCGA--CGACGGCCGC-CGCAUCc -3' miRNA: 3'- -GUUaGCUaaGCUGCCGGCGgGUGUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 45532 | 0.72 | 0.473494 |
Target: 5'- aGGUCGuaUUGACGGCCGCCgCGgcuCGUCu -3' miRNA: 3'- gUUAGCuaAGCUGCCGGCGG-GU---GUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 15014 | 0.71 | 0.525974 |
Target: 5'- aGGUCag--CGACGGCCGCCC-CuUCg -3' miRNA: 3'- gUUAGcuaaGCUGCCGGCGGGuGuAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 15330 | 0.69 | 0.624944 |
Target: 5'- -cGUCGg--CGAUGGCUGCUgCGCGUCg -3' miRNA: 3'- guUAGCuaaGCUGCCGGCGG-GUGUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 8470 | 0.68 | 0.680564 |
Target: 5'- -cAUUGA--CGAC-GCCGCCCACGUg -3' miRNA: 3'- guUAGCUaaGCUGcCGGCGGGUGUAg -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 32189 | 0.68 | 0.658381 |
Target: 5'- aGGUCGc--CGAC-GCCGCCCGCAc- -3' miRNA: 3'- gUUAGCuaaGCUGcCGGCGGGUGUag -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 36084 | 0.68 | 0.669491 |
Target: 5'- aGAUCGAcguccaCGACGGCCGCgUAUAc- -3' miRNA: 3'- gUUAGCUaa----GCUGCCGGCGgGUGUag -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 31511 | 0.68 | 0.702558 |
Target: 5'- ---cCGGccagcCGACGGCgGCCUACAUUg -3' miRNA: 3'- guuaGCUaa---GCUGCCGgCGGGUGUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 26773 | 0.67 | 0.713456 |
Target: 5'- gCGGUCGGggaaaggUUGACGGCgaaGCCCGCc-- -3' miRNA: 3'- -GUUAGCUa------AGCUGCCGg--CGGGUGuag -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 43531 | 0.67 | 0.724271 |
Target: 5'- ---aCGccgUCGGCGGCCaucGCgCGCAUCa -3' miRNA: 3'- guuaGCua-AGCUGCCGG---CGgGUGUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 11291 | 0.67 | 0.733924 |
Target: 5'- -cAUCGccaaacgUCGGCaccaugacacuuuGcGCCGCCCGCAUCg -3' miRNA: 3'- guUAGCua-----AGCUG-------------C-CGGCGGGUGUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 48477 | 0.67 | 0.756103 |
Target: 5'- uCAAUCGucagcccgCGGCGGCCGgccuUCCGCuUCa -3' miRNA: 3'- -GUUAGCuaa-----GCUGCCGGC----GGGUGuAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 19227 | 0.66 | 0.806401 |
Target: 5'- ---cCGcccgUCGACGG-CGCCCGCAc- -3' miRNA: 3'- guuaGCua--AGCUGCCgGCGGGUGUag -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 7854 | 0.66 | 0.776694 |
Target: 5'- gCAGUCGAUcUGAuaggaaccggaaCGGCCGCCa--GUCa -3' miRNA: 3'- -GUUAGCUAaGCU------------GCCGGCGGgugUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 49114 | 0.66 | 0.776694 |
Target: 5'- -cAUCGAacuuggccugcUUCGGCGuGCCGaCCCGguUCu -3' miRNA: 3'- guUAGCU-----------AAGCUGC-CGGC-GGGUguAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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