miRNA display CGI


Results 21 - 23 of 23 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12248 5' -53.6 NC_003309.1 + 23743 0.74 0.351138
Target:  5'- cCAAUCGAUcagaacagccCGACuGGCUGCgCCGCAUCg -3'
miRNA:   3'- -GUUAGCUAa---------GCUG-CCGGCG-GGUGUAG- -5'
12248 5' -53.6 NC_003309.1 + 29528 0.75 0.310436
Target:  5'- --uUCGGcagaUCGACGGCCGUCCGacCAUCg -3'
miRNA:   3'- guuAGCUa---AGCUGCCGGCGGGU--GUAG- -5'
12248 5' -53.6 NC_003309.1 + 49949 0.75 0.287789
Target:  5'- -cGUCGAUUCGGCGGCCGagCCG-GUCa -3'
miRNA:   3'- guUAGCUAAGCUGCCGGCg-GGUgUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.