Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12248 | 5' | -53.6 | NC_003309.1 | + | 43531 | 0.67 | 0.724271 |
Target: 5'- ---aCGccgUCGGCGGCCaucGCgCGCAUCa -3' miRNA: 3'- guuaGCua-AGCUGCCGG---CGgGUGUAG- -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 26773 | 0.67 | 0.713456 |
Target: 5'- gCGGUCGGggaaaggUUGACGGCgaaGCCCGCc-- -3' miRNA: 3'- -GUUAGCUa------AGCUGCCGg--CGGGUGuag -5' |
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12248 | 5' | -53.6 | NC_003309.1 | + | 49949 | 0.75 | 0.287789 |
Target: 5'- -cGUCGAUUCGGCGGCCGagCCG-GUCa -3' miRNA: 3'- guUAGCUAAGCUGCCGGCg-GGUgUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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