Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12249 | 5' | -51.3 | NC_003309.1 | + | 3327 | 0.66 | 0.885709 |
Target: 5'- aGAu--CCGcg-CGAUGUCGAGCGCg -3' miRNA: 3'- gCUuacGGCuaaGCUACGGCUUGCGa -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 9396 | 0.68 | 0.834438 |
Target: 5'- gCGGcgGUCGuAUUCGGUcguauucGCCGuAACGCUg -3' miRNA: 3'- -GCUuaCGGC-UAAGCUA-------CGGC-UUGCGA- -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 13525 | 0.66 | 0.898966 |
Target: 5'- uGAAUGUCGAUUCGcgauuguucagGCCGucUGCg -3' miRNA: 3'- gCUUACGGCUAAGCua---------CGGCuuGCGa -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 15308 | 0.67 | 0.877958 |
Target: 5'- uCGGAUGUCugcUCGAaGCCGGugGCc -3' miRNA: 3'- -GCUUACGGcuaAGCUaCGGCUugCGa -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 21902 | 0.7 | 0.701821 |
Target: 5'- uGcAUGCCGugaCGAUGCCGAuuggaucuggccGCGCg -3' miRNA: 3'- gCuUACGGCuaaGCUACGGCU------------UGCGa -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 28565 | 0.78 | 0.290692 |
Target: 5'- ----gGCCGAUUCGAUGCCaGACGUUu -3' miRNA: 3'- gcuuaCGGCUAAGCUACGGcUUGCGA- -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 30721 | 0.7 | 0.728185 |
Target: 5'- uGAAUGCCG---CGAUGauagcgacuaucgaaCCGAACGCUu -3' miRNA: 3'- gCUUACGGCuaaGCUAC---------------GGCUUGCGA- -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 32202 | 0.73 | 0.544856 |
Target: 5'- gCGAGUGCCGAUcaGGUcGCCG-ACGCc -3' miRNA: 3'- -GCUUACGGCUAagCUA-CGGCuUGCGa -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 35536 | 0.74 | 0.469988 |
Target: 5'- gGAAU-CCGAcgUCGAUGCCGAGaGCUa -3' miRNA: 3'- gCUUAcGGCUa-AGCUACGGCUUgCGA- -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 43602 | 0.66 | 0.893185 |
Target: 5'- cCGggUGCCcg-UCGGgcagGUaCGAGCGCa -3' miRNA: 3'- -GCuuACGGcuaAGCUa---CG-GCUUGCGa -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 44528 | 0.66 | 0.885709 |
Target: 5'- uGAGUucgcaGCCGAUgaaaCGGcgGCCGAgGCGCUg -3' miRNA: 3'- gCUUA-----CGGCUAa---GCUa-CGGCU-UGCGA- -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 48028 | 0.73 | 0.533889 |
Target: 5'- aGAGUgGCCGG-UCGAggucGCCGGAUGCg -3' miRNA: 3'- gCUUA-CGGCUaAGCUa---CGGCUUGCGa -5' |
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12249 | 5' | -51.3 | NC_003309.1 | + | 49457 | 0.79 | 0.261959 |
Target: 5'- gCGAAUGCCGAUUgCGAUGCaCGuAUGCc -3' miRNA: 3'- -GCUUACGGCUAA-GCUACG-GCuUGCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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