Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12250 | 3' | -48 | NC_003309.1 | + | 43352 | 0.66 | 0.982578 |
Target: 5'- --gUCUUCGAgCGUCUUgaCGAUCu- -3' miRNA: 3'- aaaAGAAGCUaGCGGAAgaGCUAGcg -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 9713 | 0.66 | 0.982578 |
Target: 5'- --gUCgagCGGUUGCCgugUgUCGcgCGCg -3' miRNA: 3'- aaaAGaa-GCUAGCGGa--AgAGCuaGCG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 5566 | 0.68 | 0.947743 |
Target: 5'- --gUCUUCGcgCGCg--C-CGAUCGCg -3' miRNA: 3'- aaaAGAAGCuaGCGgaaGaGCUAGCG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 48852 | 0.68 | 0.942712 |
Target: 5'- -cUUCa-CGAUCGCCUUCU-GA-CGCu -3' miRNA: 3'- aaAAGaaGCUAGCGGAAGAgCUaGCG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 26799 | 0.68 | 0.931732 |
Target: 5'- ----aUUCGGUCGCCUcauUCUCGugaggaGCg -3' miRNA: 3'- aaaagAAGCUAGCGGA---AGAGCuag---CG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 19860 | 0.69 | 0.919518 |
Target: 5'- -cUUUUUCGcgaccgccauGUCGCCcUCggCGAUCGCc -3' miRNA: 3'- aaAAGAAGC----------UAGCGGaAGa-GCUAGCG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 47853 | 0.69 | 0.919518 |
Target: 5'- ---gCUUCGucacGUCGCCUcCU-GAUCGCg -3' miRNA: 3'- aaaaGAAGC----UAGCGGAaGAgCUAGCG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 49844 | 0.69 | 0.912948 |
Target: 5'- -gUUCUUCGcgCGCgg-CUCGcUCGCc -3' miRNA: 3'- aaAAGAAGCuaGCGgaaGAGCuAGCG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 17842 | 0.71 | 0.821601 |
Target: 5'- aUUUCggUGAUCGCUUcgaUgUUGAUCGCg -3' miRNA: 3'- aAAAGaaGCUAGCGGA---AgAGCUAGCG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 11511 | 0.72 | 0.791466 |
Target: 5'- -cUUCUgcgacgCGAggcUCGCCgUgUCGAUCGCg -3' miRNA: 3'- aaAAGAa-----GCU---AGCGGaAgAGCUAGCG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 11799 | 0.75 | 0.622928 |
Target: 5'- --gUCggCGAUCGCCUUUUCGAgcgacuuguaCGCg -3' miRNA: 3'- aaaAGaaGCUAGCGGAAGAGCUa---------GCG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 5196 | 0.75 | 0.59969 |
Target: 5'- -cUUCUUCGccgaaGCCgcgCUCGAUCGCc -3' miRNA: 3'- aaAAGAAGCuag--CGGaa-GAGCUAGCG- -5' |
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12250 | 3' | -48 | NC_003309.1 | + | 15879 | 0.79 | 0.39461 |
Target: 5'- -----cUCGAUCGCCUUCUUGAUCu- -3' miRNA: 3'- aaaagaAGCUAGCGGAAGAGCUAGcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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