Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12253 | 5' | -54 | NC_003309.1 | + | 50160 | 0.66 | 0.7997 |
Target: 5'- cCAGCCaaCGCGAaagguucuGGAUCgcuUCGGaGCg -3' miRNA: 3'- cGUCGGagGCGCU--------UCUAGa--AGCCaCG- -5' |
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12253 | 5' | -54 | NC_003309.1 | + | 37638 | 0.66 | 0.785939 |
Target: 5'- cGCGGCCggCUGCGAuaacGUCccggccggcuacgUCGGUGUg -3' miRNA: 3'- -CGUCGGa-GGCGCUuc--UAGa------------AGCCACG- -5' |
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12253 | 5' | -54 | NC_003309.1 | + | 43639 | 0.66 | 0.759562 |
Target: 5'- cGCAGCgUCgGUuccugGccGAUCUcgCGGUGCu -3' miRNA: 3'- -CGUCGgAGgCG-----CuuCUAGAa-GCCACG- -5' |
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12253 | 5' | -54 | NC_003309.1 | + | 573 | 0.66 | 0.759562 |
Target: 5'- aGCAGCUU-CGCGAaaGGGUUgUCgGGUGUu -3' miRNA: 3'- -CGUCGGAgGCGCU--UCUAGaAG-CCACG- -5' |
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12253 | 5' | -54 | NC_003309.1 | + | 15916 | 0.67 | 0.728028 |
Target: 5'- cGCAGCaCUCgCGCGGAuacUCUgcgagCGcGUGCg -3' miRNA: 3'- -CGUCG-GAG-GCGCUUcu-AGAa----GC-CACG- -5' |
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12253 | 5' | -54 | NC_003309.1 | + | 43440 | 0.67 | 0.7065 |
Target: 5'- cGCAGCCgcgCCGCGA-GAUUgaacagcacCuGUGCg -3' miRNA: 3'- -CGUCGGa--GGCGCUuCUAGaa-------GcCACG- -5' |
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12253 | 5' | -54 | NC_003309.1 | + | 33364 | 0.67 | 0.695619 |
Target: 5'- cCAGCCUCCaCGAu--UCc-CGGUGCg -3' miRNA: 3'- cGUCGGAGGcGCUucuAGaaGCCACG- -5' |
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12253 | 5' | -54 | NC_003309.1 | + | 5057 | 0.68 | 0.684676 |
Target: 5'- gGCGGCCggugCCGCGAcggcGGUCggaUUCGG-GUc -3' miRNA: 3'- -CGUCGGa---GGCGCUu---CUAG---AAGCCaCG- -5' |
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12253 | 5' | -54 | NC_003309.1 | + | 43523 | 0.69 | 0.622762 |
Target: 5'- gGCGGCCaUCgCGCGcaucAGGUCgcggcagucuccgCGGUGCg -3' miRNA: 3'- -CGUCGG-AG-GCGCu---UCUAGaa-----------GCCACG- -5' |
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12253 | 5' | -54 | NC_003309.1 | + | 3936 | 0.69 | 0.611682 |
Target: 5'- gGCAGCCaUCUGCGcgccgcugucgAAuucgccguccuccauGAUCUUCGGcUGCg -3' miRNA: 3'- -CGUCGG-AGGCGC-----------UU---------------CUAGAAGCC-ACG- -5' |
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12253 | 5' | -54 | NC_003309.1 | + | 4988 | 0.73 | 0.400367 |
Target: 5'- aGCGGCUUCCGCGcguucGAUCUgcgCGGUc- -3' miRNA: 3'- -CGUCGGAGGCGCuu---CUAGAa--GCCAcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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