Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12254 | 5' | -50.1 | NC_003309.1 | + | 44755 | 0.66 | 0.957146 |
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Target: 5'- aGUCGaGcgCGUAUUCGAGguucugcucauCUUCGuuuCCGGu -3' miRNA: 3'- -CAGC-Ca-GCAUAAGCUC-----------GAAGUu--GGCC- -5' |
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| 12254 | 5' | -50.1 | NC_003309.1 | + | 9384 | 0.67 | 0.927208 |
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Target: 5'- uUCGGUCGUAUUCGccguaacGCUgacgaUCAGCuCGc -3' miRNA: 3'- cAGCCAGCAUAAGCu------CGA-----AGUUG-GCc -5' |
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| 12254 | 5' | -50.1 | NC_003309.1 | + | 13799 | 0.68 | 0.894325 |
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Target: 5'- -gCGGUUGUugUCGccgccGCUUCcGCCGGg -3' miRNA: 3'- caGCCAGCAuaAGCu----CGAAGuUGGCC- -5' |
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| 12254 | 5' | -50.1 | NC_003309.1 | + | 4936 | 0.68 | 0.886896 |
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Target: 5'- aGUUGGUCGaAUUCGGccuguuGC-UCGACCGa -3' miRNA: 3'- -CAGCCAGCaUAAGCU------CGaAGUUGGCc -5' |
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| 12254 | 5' | -50.1 | NC_003309.1 | + | 5080 | 0.71 | 0.757843 |
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Target: 5'- uUCGGUUGUGcaugcaCGGGCgcggCGGCCGGu -3' miRNA: 3'- cAGCCAGCAUaa----GCUCGaa--GUUGGCC- -5' |
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| 12254 | 5' | -50.1 | NC_003309.1 | + | 29598 | 0.71 | 0.725668 |
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Target: 5'- -aCGGUgG-AUUCGAGCggCGugCGGg -3' miRNA: 3'- caGCCAgCaUAAGCUCGaaGUugGCC- -5' |
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| 12254 | 5' | -50.1 | NC_003309.1 | + | 48511 | 0.74 | 0.580151 |
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Target: 5'- aUCGGUCGUcaUCGAcGCUUCGuauCCGa -3' miRNA: 3'- cAGCCAGCAuaAGCU-CGAAGUu--GGCc -5' |
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| 12254 | 5' | -50.1 | NC_003309.1 | + | 5038 | 0.76 | 0.471908 |
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Target: 5'- -gCGGUCGgAUUCGGGU--CAACCGGa -3' miRNA: 3'- caGCCAGCaUAAGCUCGaaGUUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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