Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12255 | 3' | -51.2 | NC_003309.1 | + | 8557 | 0.71 | 0.698562 |
Target: 5'- aUCUgc--GAUCGGggaguuacgCCGCCGGcGGCGGc -3' miRNA: 3'- -AGAauuuCUAGCUa--------GGCGGCC-UCGCC- -5' |
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12255 | 3' | -51.2 | NC_003309.1 | + | 15992 | 0.66 | 0.917339 |
Target: 5'- -------aAUUGGUCCGUCGGcGCGGc -3' miRNA: 3'- agaauuucUAGCUAGGCGGCCuCGCC- -5' |
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12255 | 3' | -51.2 | NC_003309.1 | + | 16171 | 0.69 | 0.803432 |
Target: 5'- ------cGAUCGAUCCGUCGGccugcacgccgAGCGa -3' miRNA: 3'- agaauuuCUAGCUAGGCGGCC-----------UCGCc -5' |
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12255 | 3' | -51.2 | NC_003309.1 | + | 28249 | 0.73 | 0.54274 |
Target: 5'- cUCUUccauAGGUCGAUCagaccaaGCCGGAGCu- -3' miRNA: 3'- -AGAAuu--UCUAGCUAGg------CGGCCUCGcc -5' |
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12255 | 3' | -51.2 | NC_003309.1 | + | 47539 | 0.67 | 0.8824 |
Target: 5'- -------cGUCGAugcagUCCGCCGGGGCa- -3' miRNA: 3'- agaauuucUAGCU-----AGGCGGCCUCGcc -5' |
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12255 | 3' | -51.2 | NC_003309.1 | + | 48020 | 0.66 | 0.923479 |
Target: 5'- ------cGGUCGAggUCGCCGGAuGCGa -3' miRNA: 3'- agaauuuCUAGCUa-GGCGGCCU-CGCc -5' |
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12255 | 3' | -51.2 | NC_003309.1 | + | 53317 | 0.66 | 0.904202 |
Target: 5'- ------cGG-CGcAUCCGCCGG-GCGGg -3' miRNA: 3'- agaauuuCUaGC-UAGGCGGCCuCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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