Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12256 | 3' | -57.2 | NC_003309.1 | + | 53125 | 0.68 | 0.451944 |
Target: 5'- -gGGCUGAcagaGGCCGcugucgCGGCGCaagaUGCGCu -3' miRNA: 3'- aaCCGACU----UCGGUa-----GCCGCGa---GUGCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 48134 | 0.68 | 0.432344 |
Target: 5'- --aGCUcGAA-CCAUCGcgaGCGCUCGCGCu -3' miRNA: 3'- aacCGA-CUUcGGUAGC---CGCGAGUGCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 47981 | 0.69 | 0.36787 |
Target: 5'- -cGGUUGAuuGCCcgugCGGCGCgccagcUCGCGCu -3' miRNA: 3'- aaCCGACUu-CGGua--GCCGCG------AGUGCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 46506 | 0.7 | 0.341504 |
Target: 5'- -aGGaucaccuuGGCCGUCGGCgggaacuGCUCGCGCa -3' miRNA: 3'- aaCCgacu----UCGGUAGCCG-------CGAGUGCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 44872 | 0.68 | 0.461923 |
Target: 5'- -cGGCUGAcucagguucGCCggCGGCGaucCGCGCa -3' miRNA: 3'- aaCCGACUu--------CGGuaGCCGCga-GUGCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 43536 | 0.66 | 0.534722 |
Target: 5'- --cGCgcacGCCGUCGGCGgccaUCGCGCg -3' miRNA: 3'- aacCGacuuCGGUAGCCGCg---AGUGCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 37249 | 0.69 | 0.359214 |
Target: 5'- -cGGCaGggGCaaggCGGCGCUacagcaGCGCg -3' miRNA: 3'- aaCCGaCuuCGgua-GCCGCGAg-----UGCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 33647 | 0.69 | 0.403901 |
Target: 5'- cUGGCUGAGGCCGgcaUCGaGCcCUCAgucgGCa -3' miRNA: 3'- aACCGACUUCGGU---AGC-CGcGAGUg---CG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 27528 | 0.66 | 0.524045 |
Target: 5'- gUGGgaUGGAGCUAUUcGCGCUCgaccaguuaaaGCGCa -3' miRNA: 3'- aACCg-ACUUCGGUAGcCGCGAG-----------UGCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 26503 | 0.67 | 0.502945 |
Target: 5'- cUGGCUGcuGCacgcagaGGCuugGCUCGCGCg -3' miRNA: 3'- aACCGACuuCGguag---CCG---CGAGUGCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 25381 | 0.68 | 0.413251 |
Target: 5'- --uGCgUGuAGCCGUCGGCGUcggUCugGCc -3' miRNA: 3'- aacCG-ACuUCGGUAGCCGCG---AGugCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 24434 | 0.66 | 0.545474 |
Target: 5'- -cGGCgGA---UAUCGGCGCUCAUGa -3' miRNA: 3'- aaCCGaCUucgGUAGCCGCGAGUGCg -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 23721 | 0.67 | 0.486335 |
Target: 5'- cUGGCUGcgccgcaucgucccaAcugaaaaugaucAGCUcgCGGCGCUCgACGCc -3' miRNA: 3'- aACCGAC---------------U------------UCGGuaGCCGCGAG-UGCG- -5' |
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12256 | 3' | -57.2 | NC_003309.1 | + | 12660 | 0.66 | 0.578113 |
Target: 5'- -cGGcCUGcaaugcGAGCCGcugcUCGagauagccGCGCUCGCGCg -3' miRNA: 3'- aaCC-GAC------UUCGGU----AGC--------CGCGAGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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