Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12259 | 3' | -58 | NC_003309.1 | + | 18419 | 0.77 | 0.123888 |
Target: 5'- gCUGaCGAGCGCGACCGUGuagcgcgucuuGCGCUCGc -3' miRNA: 3'- -GACaGCUCGUGUUGGCGC-----------CGCGAGCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 9519 | 0.75 | 0.168953 |
Target: 5'- -cGUCGAGCGCGcgcACCgugaccugcacgcuuGCGGCGUUCGa -3' miRNA: 3'- gaCAGCUCGUGU---UGG---------------CGCCGCGAGCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 18732 | 0.73 | 0.217753 |
Target: 5'- uUGUCGAGCGugaucgucucgcCGGCUGCGGCGCggauaCGg -3' miRNA: 3'- gACAGCUCGU------------GUUGGCGCCGCGa----GCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 1794 | 0.72 | 0.241422 |
Target: 5'- --uUCGGGCACccagAACCGCGGCGCa--- -3' miRNA: 3'- gacAGCUCGUG----UUGGCGCCGCGagca -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 12004 | 0.71 | 0.305458 |
Target: 5'- -cGUCGAaagccugcugcccgcGCGCGgcaGCCGCGGCGCgcgccgCGUc -3' miRNA: 3'- gaCAGCU---------------CGUGU---UGGCGCCGCGa-----GCA- -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 3316 | 0.71 | 0.30996 |
Target: 5'- aUGUCGAGCGCGGaaaggCGCaGCGCgUCGa -3' miRNA: 3'- gACAGCUCGUGUUg----GCGcCGCG-AGCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 32016 | 0.7 | 0.317574 |
Target: 5'- aCUGgggacCGGGCGC-ACCGCGGCG-UCGc -3' miRNA: 3'- -GACa----GCUCGUGuUGGCGCCGCgAGCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 19944 | 0.7 | 0.344509 |
Target: 5'- --uUCGAcgcGCGCGaucgcuuccguuugcACCGCGGCGCUCa- -3' miRNA: 3'- gacAGCU---CGUGU---------------UGGCGCCGCGAGca -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 11843 | 0.69 | 0.383496 |
Target: 5'- uUGUCGAuguugguucGCGCGcccGCCGCGGCuuUCGa -3' miRNA: 3'- gACAGCU---------CGUGU---UGGCGCCGcgAGCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 16276 | 0.69 | 0.39235 |
Target: 5'- -aGUCGcGCACGAUCGCGuaGCagUCGUg -3' miRNA: 3'- gaCAGCuCGUGUUGGCGCcgCG--AGCA- -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 27482 | 0.68 | 0.448166 |
Target: 5'- -aGUCGAGCGCGGCgaaGaGGCGUUCu- -3' miRNA: 3'- gaCAGCUCGUGUUGg--CgCCGCGAGca -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 5148 | 0.68 | 0.448166 |
Target: 5'- -cGUCGccGCGCGccGCCGCGaGCGCaCGUa -3' miRNA: 3'- gaCAGCu-CGUGU--UGGCGC-CGCGaGCA- -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 44280 | 0.68 | 0.457891 |
Target: 5'- uCUGcCGcuuuGCACGagcGCCGCGGUuguGCUCGa -3' miRNA: 3'- -GACaGCu---CGUGU---UGGCGCCG---CGAGCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 43272 | 0.67 | 0.487721 |
Target: 5'- -cGUCGcAGCACu-CaaCGGCGCUCGg -3' miRNA: 3'- gaCAGC-UCGUGuuGgcGCCGCGAGCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 46312 | 0.67 | 0.487721 |
Target: 5'- -cGcCGAGCACGGCCuucugcgcacGCGaGCGCgCGUg -3' miRNA: 3'- gaCaGCUCGUGUUGG----------CGC-CGCGaGCA- -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 31421 | 0.67 | 0.518435 |
Target: 5'- gCUGgCGAccaguGCGCAGCCGCcGGCGUgcCGUu -3' miRNA: 3'- -GACaGCU-----CGUGUUGGCG-CCGCGa-GCA- -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 10727 | 0.67 | 0.518435 |
Target: 5'- gCUGcgCGAGCGCGagcgaaGCCuCGGCGCgcUCGa -3' miRNA: 3'- -GACa-GCUCGUGU------UGGcGCCGCG--AGCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 17304 | 0.66 | 0.549887 |
Target: 5'- --aUCGAGCACcuUCGCGcGCGCaaUCGa -3' miRNA: 3'- gacAGCUCGUGuuGGCGC-CGCG--AGCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 16003 | 0.66 | 0.560506 |
Target: 5'- aUG-CGAGCGCGAauugguCCGuCGGCGCggcugCGa -3' miRNA: 3'- gACaGCUCGUGUU------GGC-GCCGCGa----GCa -5' |
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12259 | 3' | -58 | NC_003309.1 | + | 1292 | 0.66 | 0.566905 |
Target: 5'- gCUGUCGAcGCGCGcccaugccagucaGCauuguuucguacagCGCGGCGCugUCGUg -3' miRNA: 3'- -GACAGCU-CGUGU-------------UG--------------GCGCCGCG--AGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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