Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12263 | 5' | -57.9 | NC_003309.1 | + | 53258 | 0.81 | 0.056797 |
Target: 5'- -cGCGCGCCGucuUCGCCGCGUGaCAGUc -3' miRNA: 3'- ucUGCGCGGUu--AGCGGCGCAC-GUCAc -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 5559 | 0.71 | 0.285073 |
Target: 5'- -cGCGCGCCGAUCGCgGCgGUGaGGUc -3' miRNA: 3'- ucUGCGCGGUUAGCGgCG-CACgUCAc -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 22632 | 0.71 | 0.2691 |
Target: 5'- uGGAaGCGCCGGgcaCGCCGUcgccaccggcugccGUGCAGUGu -3' miRNA: 3'- -UCUgCGCGGUUa--GCGGCG--------------CACGUCAC- -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 5064 | 0.69 | 0.389307 |
Target: 5'- cGGGCGCggcgGCCGGU-GCCGCGacgGCGGUc -3' miRNA: 3'- -UCUGCG----CGGUUAgCGGCGCa--CGUCAc -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 17820 | 0.69 | 0.36316 |
Target: 5'- uGAuCGCGUCGgcgaucGUCGCCGUGUGCGcGUu -3' miRNA: 3'- uCU-GCGCGGU------UAGCGGCGCACGU-CAc -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 48710 | 0.68 | 0.445151 |
Target: 5'- cGAC-UGCCAAU-GCCGCGUGCGc-- -3' miRNA: 3'- uCUGcGCGGUUAgCGGCGCACGUcac -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 10318 | 0.68 | 0.404662 |
Target: 5'- cGGGCGCcgacccacgcugucGCCGccugcGUCGCCGCGacUGCGGa- -3' miRNA: 3'- -UCUGCG--------------CGGU-----UAGCGGCGC--ACGUCac -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 9187 | 0.67 | 0.494937 |
Target: 5'- -aACGcCGCCGccgcgaucAUCGCCGCaUGCAGa- -3' miRNA: 3'- ucUGC-GCGGU--------UAGCGGCGcACGUCac -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 45264 | 0.66 | 0.568471 |
Target: 5'- uGGuCGaauGCCGGcCGCCGCGUGCGc-- -3' miRNA: 3'- -UCuGCg--CGGUUaGCGGCGCACGUcac -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 27743 | 0.66 | 0.568471 |
Target: 5'- cGACGCGCCGcccggcgcAUCGCCGaaauCAGUc -3' miRNA: 3'- uCUGCGCGGU--------UAGCGGCgcacGUCAc -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 9580 | 0.66 | 0.557757 |
Target: 5'- uGAUGCacGCCGAaCGCCGC-UGCGGc- -3' miRNA: 3'- uCUGCG--CGGUUaGCGGCGcACGUCac -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 36446 | 0.66 | 0.536512 |
Target: 5'- aAGAgCGCGUCAAg-GCCGCGcUGCaAGUc -3' miRNA: 3'- -UCU-GCGCGGUUagCGGCGC-ACG-UCAc -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 30800 | 0.66 | 0.536512 |
Target: 5'- uGGCGCGCaaugucgCGCCGCGUcuGCAa-- -3' miRNA: 3'- uCUGCGCGguua---GCGGCGCA--CGUcac -5' |
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12263 | 5' | -57.9 | NC_003309.1 | + | 19942 | 0.66 | 0.525994 |
Target: 5'- cGACGCGCgCGAUCGCuuccguuugcacCGCGgcgcucaGCAGg- -3' miRNA: 3'- uCUGCGCG-GUUAGCG------------GCGCa------CGUCac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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