Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12264 | 5' | -53 | NC_003309.1 | + | 12019 | 0.74 | 0.388453 |
Target: 5'- uGGAC-GCGAUCGucucGUCGAaagccuGCUGCCCGc -3' miRNA: 3'- gCUUGaCGCUAGU----UAGCU------CGACGGGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 12835 | 0.74 | 0.388453 |
Target: 5'- gCGAucauGCUGuCGAUCAGUCcgcggaaauuGAGCUuGCCCGa -3' miRNA: 3'- -GCU----UGAC-GCUAGUUAG----------CUCGA-CGGGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 4252 | 0.72 | 0.496959 |
Target: 5'- -cGGCUGCGAUCAGgu-AGCcGCCCGa -3' miRNA: 3'- gcUUGACGCUAGUUagcUCGaCGGGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 9534 | 0.72 | 0.496959 |
Target: 5'- uCGAACUGCGcgCcgcGUCGAGCgcgcGCaCCGu -3' miRNA: 3'- -GCUUGACGCuaGu--UAGCUCGa---CG-GGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 4625 | 0.72 | 0.507517 |
Target: 5'- gCGAACUucggcaGCGAUgCGAUUGAuuGCUGCCUGu -3' miRNA: 3'- -GCUUGA------CGCUA-GUUAGCU--CGACGGGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 35822 | 0.7 | 0.583676 |
Target: 5'- uCGAGCUGguCGGcgCAAUCGAcuuaCUGCCCGa -3' miRNA: 3'- -GCUUGAC--GCUa-GUUAGCUc---GACGGGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 26133 | 0.68 | 0.738487 |
Target: 5'- aGGACUGCGGUUAaguaGUUGAcGCagggaacGCCCGa -3' miRNA: 3'- gCUUGACGCUAGU----UAGCU-CGa------CGGGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 46484 | 0.67 | 0.780133 |
Target: 5'- gGAACUGCucgcgcauaGcgCGGUUGAGgUGCUCGa -3' miRNA: 3'- gCUUGACG---------CuaGUUAGCUCgACGGGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 40232 | 0.67 | 0.790178 |
Target: 5'- aCGGaaGCUGcCGAccgugUCGGUCGAGUgGCUCGa -3' miRNA: 3'- -GCU--UGAC-GCU-----AGUUAGCUCGaCGGGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 1706 | 0.66 | 0.803953 |
Target: 5'- uCGAGCuUGCGcgCcauGUCGAGCgcgagcacgcaaucuUGCCCc -3' miRNA: 3'- -GCUUG-ACGCuaGu--UAGCUCG---------------ACGGGc -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 48248 | 0.66 | 0.819248 |
Target: 5'- aCGuauCUGaCGGcucgcUCGAUCGAGCcGUCCGa -3' miRNA: 3'- -GCuu-GAC-GCU-----AGUUAGCUCGaCGGGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 48008 | 0.66 | 0.828546 |
Target: 5'- cCGGA-UGCGAUUucgucgAAUCGAGUucgguugauUGCCCGu -3' miRNA: 3'- -GCUUgACGCUAG------UUAGCUCG---------ACGGGC- -5' |
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12264 | 5' | -53 | NC_003309.1 | + | 23437 | 0.66 | 0.846492 |
Target: 5'- uCGAGCagGuCGccCAAUCGAGCcGCUCGa -3' miRNA: 3'- -GCUUGa-C-GCuaGUUAGCUCGaCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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