Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12265 | 3' | -61.5 | NC_003309.1 | + | 46787 | 0.66 | 0.366466 |
Target: 5'- cGCgUgGCCGcuGCGCGAGCUGucacCGCa -3' miRNA: 3'- uCGaAgCGGCu-CGCGCUCGACc---GCGc -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 32968 | 0.66 | 0.383466 |
Target: 5'- cGCg-CGCUGAgGCGCGAGa--GCGCGa -3' miRNA: 3'- uCGaaGCGGCU-CGCGCUCgacCGCGC- -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 31932 | 0.66 | 0.400983 |
Target: 5'- -uCUUCGCCGccCGCG-GCcGGCGCc -3' miRNA: 3'- ucGAAGCGGCucGCGCuCGaCCGCGc -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 28201 | 0.68 | 0.2825 |
Target: 5'- -uCUUCGUCagGGGCGCGAuaGUUGGUGCa -3' miRNA: 3'- ucGAAGCGG--CUCGCGCU--CGACCGCGc -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 22441 | 0.69 | 0.225742 |
Target: 5'- cGCUgccggCGUCGAGCGCG-GC--GCGCGa -3' miRNA: 3'- uCGAa----GCGGCUCGCGCuCGacCGCGC- -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 22059 | 0.67 | 0.341953 |
Target: 5'- aAGCUcacCGCCGc-CGCGAGCUGauauGUGCGc -3' miRNA: 3'- -UCGAa--GCGGCucGCGCUCGAC----CGCGC- -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 19306 | 0.71 | 0.165379 |
Target: 5'- cGUcaCGCCGAGCGCGuuUUcGGCGCGc -3' miRNA: 3'- uCGaaGCGGCUCGCGCucGA-CCGCGC- -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 18739 | 0.7 | 0.198586 |
Target: 5'- cGGCgccUUGUCGAGCGUGAucgucucgccgGCUgcGGCGCGg -3' miRNA: 3'- -UCGa--AGCGGCUCGCGCU-----------CGA--CCGCGC- -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 17811 | 0.7 | 0.198586 |
Target: 5'- cGGCgaucgUCGCCGuGUGCGcGUUcGGCGUGa -3' miRNA: 3'- -UCGa----AGCGGCuCGCGCuCGA-CCGCGC- -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 16927 | 0.66 | 0.358162 |
Target: 5'- cGCgaucgCGCCGAcaGCGaCGAGUgcGGCGCc -3' miRNA: 3'- uCGaa---GCGGCU--CGC-GCUCGa-CCGCGc -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 12863 | 0.66 | 0.392161 |
Target: 5'- cGCgcgCGCCGAaaaccGCGCGAGgUcGGCGa- -3' miRNA: 3'- uCGaa-GCGGCU-----CGCGCUCgA-CCGCgc -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 10731 | 0.75 | 0.088719 |
Target: 5'- cGCUgcuGCgCGAGCGCGAGCgaagccucGGCGCGc -3' miRNA: 3'- uCGAag-CG-GCUCGCGCUCGa-------CCGCGC- -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 10328 | 0.66 | 0.349991 |
Target: 5'- gAGCgcUCGCCGGGCGCcGAcccacGCUGuCGCc -3' miRNA: 3'- -UCGa-AGCGGCUCGCG-CU-----CGACcGCGc -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 8544 | 0.66 | 0.383466 |
Target: 5'- gAGUUaCGCCGccggcGGCGgcaCGAGCUucGGCGCa -3' miRNA: 3'- -UCGAaGCGGC-----UCGC---GCUCGA--CCGCGc -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 6361 | 0.68 | 0.289461 |
Target: 5'- -cCUUCGCCGcG-GUGGGCuUGGCGCu -3' miRNA: 3'- ucGAAGCGGCuCgCGCUCG-ACCGCGc -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 5179 | 0.66 | 0.349991 |
Target: 5'- cGCUcgaUCGCCaguuucGAG-GCGAGCUG-CGCGu -3' miRNA: 3'- uCGA---AGCGG------CUCgCGCUCGACcGCGC- -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 3320 | 0.66 | 0.358162 |
Target: 5'- cGCgaUGUCGAGCGCGGaaaGGCGCa -3' miRNA: 3'- uCGaaGCGGCUCGCGCUcgaCCGCGc -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 2480 | 0.66 | 0.392161 |
Target: 5'- -cCUUCGCUGAacgacucgcgcaGCGCGAGCcGcCGCGa -3' miRNA: 3'- ucGAAGCGGCU------------CGCGCUCGaCcGCGC- -5' |
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12265 | 3' | -61.5 | NC_003309.1 | + | 1704 | 0.67 | 0.326282 |
Target: 5'- gAGCUUgcgcgccaUGUCGAGCGCGAGCa--CGCa -3' miRNA: 3'- -UCGAA--------GCGGCUCGCGCUCGaccGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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