Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12265 | 5' | -46.7 | NC_003309.1 | + | 35304 | 0.66 | 0.991787 |
Target: 5'- -cCuAUCGGGCggGGUUCGACGAAGg -3' miRNA: 3'- uaGuUGGCUUGagCUAAGCUGCUUUa -5' |
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12265 | 5' | -46.7 | NC_003309.1 | + | 1010 | 0.66 | 0.990442 |
Target: 5'- ---cACCGAACUCGucgUCGAgGAc-- -3' miRNA: 3'- uaguUGGCUUGAGCua-AGCUgCUuua -5' |
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12265 | 5' | -46.7 | NC_003309.1 | + | 36479 | 0.66 | 0.990442 |
Target: 5'- uUCAcUCGuACUCGAccUUCGGCGAGc- -3' miRNA: 3'- uAGUuGGCuUGAGCU--AAGCUGCUUua -5' |
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12265 | 5' | -46.7 | NC_003309.1 | + | 13288 | 0.67 | 0.980954 |
Target: 5'- -cCAACCGcgaucaGGCUCGcaggGUUCGACGAu-- -3' miRNA: 3'- uaGUUGGC------UUGAGC----UAAGCUGCUuua -5' |
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12265 | 5' | -46.7 | NC_003309.1 | + | 21745 | 0.67 | 0.978416 |
Target: 5'- -gCAG-CGAGCUCGAUcugcucUCGACGAu-- -3' miRNA: 3'- uaGUUgGCUUGAGCUA------AGCUGCUuua -5' |
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12265 | 5' | -46.7 | NC_003309.1 | + | 48278 | 0.67 | 0.975636 |
Target: 5'- -cCAGCCGAAUUgCGAUaggugaagUCGGCGAc-- -3' miRNA: 3'- uaGUUGGCUUGA-GCUA--------AGCUGCUuua -5' |
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12265 | 5' | -46.7 | NC_003309.1 | + | 17655 | 0.68 | 0.957308 |
Target: 5'- cGUCGACCGAcaAgUCGAUcgcauagUCGACGcGAUa -3' miRNA: 3'- -UAGUUGGCU--UgAGCUA-------AGCUGCuUUA- -5' |
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12265 | 5' | -46.7 | NC_003309.1 | + | 8974 | 0.69 | 0.943496 |
Target: 5'- cAUCGagcuuGCCGAACUCGAgcgccuucaCGACGAu-- -3' miRNA: 3'- -UAGU-----UGGCUUGAGCUaa-------GCUGCUuua -5' |
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12265 | 5' | -46.7 | NC_003309.1 | + | 12602 | 0.7 | 0.932444 |
Target: 5'- cUCGGCCGuguugaugcGCUCGAUcUCGGCGAu-- -3' miRNA: 3'- uAGUUGGCu--------UGAGCUA-AGCUGCUuua -5' |
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12265 | 5' | -46.7 | NC_003309.1 | + | 15579 | 0.7 | 0.920121 |
Target: 5'- ---cACCGAAgUCGAgcggCGACGAGAg -3' miRNA: 3'- uaguUGGCUUgAGCUaa--GCUGCUUUa -5' |
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12265 | 5' | -46.7 | NC_003309.1 | + | 20173 | 0.71 | 0.891689 |
Target: 5'- -cCGACaCGAGCaccugCGAUUCGACGAc-- -3' miRNA: 3'- uaGUUG-GCUUGa----GCUAAGCUGCUuua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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