Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12266 | 3' | -63.1 | NC_003309.1 | + | 46757 | 0.66 | 0.337882 |
Target: 5'- aCGgGCGUCacuucgccAGCGcGCGC-CGGAacgGGCUc -3' miRNA: 3'- -GCgCGCAG--------UCGC-CGCGaGCCUg--CCGA- -5' |
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12266 | 3' | -63.1 | NC_003309.1 | + | 37278 | 0.66 | 0.329433 |
Target: 5'- gGCGCGUCGaUGGCGgUCGaGgacgucuACGGCa -3' miRNA: 3'- gCGCGCAGUcGCCGCgAGC-C-------UGCCGa -5' |
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12266 | 3' | -63.1 | NC_003309.1 | + | 45242 | 0.66 | 0.315208 |
Target: 5'- uGCGCcucgaauacGUCGGCGGCaCUCcagcgcagaaugGGGCGGUa -3' miRNA: 3'- gCGCG---------CAGUCGCCGcGAG------------CCUGCCGa -5' |
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12266 | 3' | -63.1 | NC_003309.1 | + | 15336 | 0.67 | 0.273359 |
Target: 5'- cCGCaGCGUCGGCGauggcugcuGCGCgUCGGAUGucuGCUc -3' miRNA: 3'- -GCG-CGCAGUCGC---------CGCG-AGCCUGC---CGA- -5' |
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12266 | 3' | -63.1 | NC_003309.1 | + | 10045 | 0.68 | 0.260435 |
Target: 5'- gCGCGCGUCAGC-GC-CUCGacgagauuaccGACGGUc -3' miRNA: 3'- -GCGCGCAGUCGcCGcGAGC-----------CUGCCGa -5' |
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12266 | 3' | -63.1 | NC_003309.1 | + | 17817 | 0.68 | 0.256656 |
Target: 5'- -uCGCGUCGGCGaucgucgccgugugcGCGUUCGGcgugauACGGCg -3' miRNA: 3'- gcGCGCAGUCGC---------------CGCGAGCC------UGCCGa -5' |
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12266 | 3' | -63.1 | NC_003309.1 | + | 11985 | 0.68 | 0.254161 |
Target: 5'- gCGCGCGgcagCcGCGGCGCgcgcCGcGuCGGCg -3' miRNA: 3'- -GCGCGCa---GuCGCCGCGa---GC-CuGCCGa -5' |
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12266 | 3' | -63.1 | NC_003309.1 | + | 47964 | 0.69 | 0.208363 |
Target: 5'- gGCGCGcCAGCucGCGCUCGG-CGaaGCUg -3' miRNA: 3'- gCGCGCaGUCGc-CGCGAGCCuGC--CGA- -5' |
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12266 | 3' | -63.1 | NC_003309.1 | + | 22385 | 0.7 | 0.188251 |
Target: 5'- uGCGCGUCGaacgcccgcGCGGCcuGCUCGu-CGGCa -3' miRNA: 3'- gCGCGCAGU---------CGCCG--CGAGCcuGCCGa -5' |
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12266 | 3' | -63.1 | NC_003309.1 | + | 5062 | 0.72 | 0.120763 |
Target: 5'- gGCGCGgCGGcCGGUGC-CGcGACGGCg -3' miRNA: 3'- gCGCGCaGUC-GCCGCGaGC-CUGCCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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