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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 12270 | 5' | -51.4 | NC_003309.1 | + | 39035 | 0.73 | 0.499318 |
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Target: 5'- --cGGacauugCCUCGACGCAAUCgaGCu -3' miRNA: 3'- cuaCCgaaa--GGAGCUGCGUUAGgaCG- -5' |
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| 12270 | 5' | -51.4 | NC_003309.1 | + | 10715 | 0.68 | 0.807433 |
|
Target: 5'- --cGaGCgaagCCUCGGCGCGcucgagcUCCUGCu -3' miRNA: 3'- cuaC-CGaaa-GGAGCUGCGUu------AGGACG- -5' |
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| 12270 | 5' | -51.4 | NC_003309.1 | + | 18952 | 0.68 | 0.807433 |
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Target: 5'- -cUGGCcgUUgUCGuCGCucUCCUGCa -3' miRNA: 3'- cuACCGaaAGgAGCuGCGuuAGGACG- -5' |
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| 12270 | 5' | -51.4 | NC_003309.1 | + | 48633 | 0.68 | 0.787395 |
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Target: 5'- uGUGGCgaUUCCgUCGGCGCcggaaGGUCCUugaGCa -3' miRNA: 3'- cUACCGa-AAGG-AGCUGCG-----UUAGGA---CG- -5' |
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| 12270 | 5' | -51.4 | NC_003309.1 | + | 7410 | 0.66 | 0.894145 |
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Target: 5'- --cGGUcUUCUUCGGCGCGcgCUUacGCg -3' miRNA: 3'- cuaCCGaAAGGAGCUGCGUuaGGA--CG- -5' |
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| 12270 | 5' | -51.4 | NC_003309.1 | + | 42235 | 0.66 | 0.862468 |
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Target: 5'- cAUGGCagUUUCUUCGACGUAgagGUUC-GCg -3' miRNA: 3'- cUACCG--AAAGGAGCUGCGU---UAGGaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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