miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12270 5' -51.4 NC_003309.1 + 39035 0.73 0.499318
Target:  5'- --cGGacauugCCUCGACGCAAUCgaGCu -3'
miRNA:   3'- cuaCCgaaa--GGAGCUGCGUUAGgaCG- -5'
12270 5' -51.4 NC_003309.1 + 10715 0.68 0.807433
Target:  5'- --cGaGCgaagCCUCGGCGCGcucgagcUCCUGCu -3'
miRNA:   3'- cuaC-CGaaa-GGAGCUGCGUu------AGGACG- -5'
12270 5' -51.4 NC_003309.1 + 18952 0.68 0.807433
Target:  5'- -cUGGCcgUUgUCGuCGCucUCCUGCa -3'
miRNA:   3'- cuACCGaaAGgAGCuGCGuuAGGACG- -5'
12270 5' -51.4 NC_003309.1 + 48633 0.68 0.787395
Target:  5'- uGUGGCgaUUCCgUCGGCGCcggaaGGUCCUugaGCa -3'
miRNA:   3'- cUACCGa-AAGG-AGCUGCG-----UUAGGA---CG- -5'
12270 5' -51.4 NC_003309.1 + 7410 0.66 0.894145
Target:  5'- --cGGUcUUCUUCGGCGCGcgCUUacGCg -3'
miRNA:   3'- cuaCCGaAAGGAGCUGCGUuaGGA--CG- -5'
12270 5' -51.4 NC_003309.1 + 42235 0.66 0.862468
Target:  5'- cAUGGCagUUUCUUCGACGUAgagGUUC-GCg -3'
miRNA:   3'- cUACCG--AAAGGAGCUGCGU---UAGGaCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.