Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12271 | 3' | -55.1 | NC_003309.1 | + | 11902 | 0.66 | 0.687027 |
Target: 5'- cGGCGAGCGCcuccgcaaccaccAUCUgcUGauUCAUcaGCGCGa -3' miRNA: 3'- -CCGCUCGCG-------------UAGA--ACgcAGUA--CGCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 15442 | 0.66 | 0.676012 |
Target: 5'- cGGCGucgagguAGCGCG-CgaGUGUCcgGCGCc -3' miRNA: 3'- -CCGC-------UCGCGUaGaaCGCAGuaCGCGu -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 42787 | 0.66 | 0.676012 |
Target: 5'- gGGCGAaaacaugGCGCuUCUUGCaGUCGuUGaCGCu -3' miRNA: 3'- -CCGCU-------CGCGuAGAACG-CAGU-AC-GCGu -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 5132 | 0.66 | 0.666061 |
Target: 5'- cGCGAGCGCA-----CGUacCAUGCGCGc -3' miRNA: 3'- cCGCUCGCGUagaacGCA--GUACGCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 19301 | 0.66 | 0.666061 |
Target: 5'- cGcCGAGCGCGUUUucgGCGcgCAcGCGCGc -3' miRNA: 3'- cC-GCUCGCGUAGAa--CGCa-GUaCGCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 25761 | 0.66 | 0.700174 |
Target: 5'- cGCgGAGCGCAugaUCggcgacggagggaucUGCGUCAUGuCGUAg -3' miRNA: 3'- cCG-CUCGCGU---AGa--------------ACGCAGUAC-GCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 10489 | 0.67 | 0.642754 |
Target: 5'- cGCGAggcuuucGCGCAUCgcGCG-CAUGcCGCGa -3' miRNA: 3'- cCGCU-------CGCGUAGaaCGCaGUAC-GCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 18404 | 0.68 | 0.54457 |
Target: 5'- cGUGuAGCGCGUCUUGCGcUCGcUGC-CGa -3' miRNA: 3'- cCGC-UCGCGUAGAACGC-AGU-ACGcGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 17811 | 0.68 | 0.566357 |
Target: 5'- cGGCGAucgucgccguguGCGCGUUcgGCGUgAUacgGCGCAc -3' miRNA: 3'- -CCGCU------------CGCGUAGaaCGCAgUA---CGCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 46657 | 0.68 | 0.577336 |
Target: 5'- gGGCcGGCGCGUaaUGCG-CA-GCGCAc -3' miRNA: 3'- -CCGcUCGCGUAgaACGCaGUaCGCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 31915 | 0.68 | 0.577336 |
Target: 5'- cGGCGccAGCGCGUCUuuUGCucGUCGcuUGCGg- -3' miRNA: 3'- -CCGC--UCGCGUAGA--ACG--CAGU--ACGCgu -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 11208 | 0.68 | 0.599425 |
Target: 5'- cGCGA-CGCcUCcUGCuUCGUGCGCAg -3' miRNA: 3'- cCGCUcGCGuAGaACGcAGUACGCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 16002 | 0.69 | 0.533778 |
Target: 5'- uGCGAGCGCGaaUUGgucCGUCG-GCGCGg -3' miRNA: 3'- cCGCUCGCGUagAAC---GCAGUaCGCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 10948 | 0.69 | 0.523062 |
Target: 5'- cGCGAGCGcCGUC-UGCGcCGUcgccGCGCu -3' miRNA: 3'- cCGCUCGC-GUAGaACGCaGUA----CGCGu -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 17725 | 0.69 | 0.506094 |
Target: 5'- uGCGAGCGCucguagagcgacgucGUCUUGCcGUCGaaCGCAc -3' miRNA: 3'- cCGCUCGCG---------------UAGAACG-CAGUacGCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 8842 | 0.7 | 0.481099 |
Target: 5'- cGCGAacgaacagccGCGCAUucgCUUGCGU-GUGCGCGu -3' miRNA: 3'- cCGCU----------CGCGUA---GAACGCAgUACGCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 10377 | 0.7 | 0.431076 |
Target: 5'- uGGCGAGCGCcgCcUGCGccgccUCGUGuUGCu -3' miRNA: 3'- -CCGCUCGCGuaGaACGC-----AGUAC-GCGu -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 34332 | 0.7 | 0.431076 |
Target: 5'- uGGCGAGCGCGUUUUuCGcUCAgGCGgAg -3' miRNA: 3'- -CCGCUCGCGUAGAAcGC-AGUaCGCgU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 7813 | 0.71 | 0.42144 |
Target: 5'- cGGCGAGCGCcauGUCgagcGCGcCAUguacGCGCGu -3' miRNA: 3'- -CCGCUCGCG---UAGaa--CGCaGUA----CGCGU- -5' |
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12271 | 3' | -55.1 | NC_003309.1 | + | 14490 | 0.71 | 0.42144 |
Target: 5'- uGCG-GCGUAUCaaGCGUCAucgguUGUGCAa -3' miRNA: 3'- cCGCuCGCGUAGaaCGCAGU-----ACGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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