Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12271 | 5' | -54.5 | NC_003309.1 | + | 5618 | 0.66 | 0.779848 |
Target: 5'- aGCgCAGGCcCuu-CGGGGUGuUCGCCg -3' miRNA: 3'- -CG-GUUCGaGcuuGUCCCACuAGCGGg -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 4164 | 0.66 | 0.759554 |
Target: 5'- cGCC-AGCUCGAACGcGccGGUCGCgCu -3' miRNA: 3'- -CGGuUCGAGCUUGUcCcaCUAGCGgG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 1972 | 0.66 | 0.749204 |
Target: 5'- cGCCGugcGGgUCGAGCGGaGUGcAUUGCaCCg -3' miRNA: 3'- -CGGU---UCgAGCUUGUCcCAC-UAGCG-GG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 28706 | 0.66 | 0.738734 |
Target: 5'- cGCCGGGCUuaugcaugCGcGACAugauGG-GAUCGCCCu -3' miRNA: 3'- -CGGUUCGA--------GC-UUGUc---CCaCUAGCGGG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 15707 | 0.67 | 0.69589 |
Target: 5'- aUCGAGCgCGGgaucgcucACGGGGUuGUCGUCCu -3' miRNA: 3'- cGGUUCGaGCU--------UGUCCCAcUAGCGGG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 28479 | 0.67 | 0.69589 |
Target: 5'- cGCCAcgccGGCcCGuAUAGcGGcGAUUGCCCg -3' miRNA: 3'- -CGGU----UCGaGCuUGUC-CCaCUAGCGGG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 32773 | 0.67 | 0.69589 |
Target: 5'- -aCAGGCUCGGACAGGacag-CGCUg -3' miRNA: 3'- cgGUUCGAGCUUGUCCcacuaGCGGg -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 8288 | 0.67 | 0.680622 |
Target: 5'- aGCCGAGCUCGGAUuccuuuucGUCGCUCg -3' miRNA: 3'- -CGGUUCGAGCUUGucccac--UAGCGGG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 40561 | 0.68 | 0.652049 |
Target: 5'- aGCCGAGa---AACGGGGUag-CGCCCg -3' miRNA: 3'- -CGGUUCgagcUUGUCCCAcuaGCGGG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 50835 | 0.68 | 0.64212 |
Target: 5'- cGCCAGGCgguauuacuaucACAGGGUGAaugaCGCCa -3' miRNA: 3'- -CGGUUCGagcu--------UGUCCCACUa---GCGGg -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 4417 | 0.68 | 0.618936 |
Target: 5'- cGCuCAgcGGCUCauGCGGGGUGAgaUCGUUCu -3' miRNA: 3'- -CG-GU--UCGAGcuUGUCCCACU--AGCGGG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 41091 | 0.68 | 0.618936 |
Target: 5'- cGCCc-GC-CccGCAGGGUGA-CGCCCc -3' miRNA: 3'- -CGGuuCGaGcuUGUCCCACUaGCGGG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 13865 | 0.68 | 0.607908 |
Target: 5'- cCCGAuaUCGAGCGGGGUcGAgCGUUCg -3' miRNA: 3'- cGGUUcgAGCUUGUCCCA-CUaGCGGG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 13279 | 0.72 | 0.42042 |
Target: 5'- aUCAGGCUC--GCAGGGUucgacGAUCcGCCCu -3' miRNA: 3'- cGGUUCGAGcuUGUCCCA-----CUAG-CGGG- -5' |
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12271 | 5' | -54.5 | NC_003309.1 | + | 31528 | 0.73 | 0.349688 |
Target: 5'- cGCC-AGCUCGAACuacgccggccagccGacggcggccuacauuGGGUGAUCGCCg -3' miRNA: 3'- -CGGuUCGAGCUUG--------------U---------------CCCACUAGCGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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