miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12282 3' -49.7 NC_003313.1 + 20101 0.67 0.815342
Target:  5'- cUCAgaccuUGAUGacguguUAGCCGaaGCCAGCGCCg -3'
miRNA:   3'- aAGU-----ACUAC------AUUGGUa-CGGUUGCGGg -5'
12282 3' -49.7 NC_003313.1 + 914 0.67 0.794471
Target:  5'- cUUUAUGAUG-AACCGUGgCGACaUCCu -3'
miRNA:   3'- -AAGUACUACaUUGGUACgGUUGcGGG- -5'
12282 3' -49.7 NC_003313.1 + 6479 0.67 0.772789
Target:  5'- --gGUGGagaGUAGCCAUGCaguACGCCg -3'
miRNA:   3'- aagUACUa--CAUUGGUACGgu-UGCGGg -5'
12282 3' -49.7 NC_003313.1 + 24049 0.67 0.770581
Target:  5'- ----aGAUGUGGCaggcaugacgGCCAGCGCCg -3'
miRNA:   3'- aaguaCUACAUUGgua-------CGGUUGCGGg -5'
12282 3' -49.7 NC_003313.1 + 20356 0.7 0.608186
Target:  5'- -cCGUGAaGUcggcguACUGUGCCAACuGCCCg -3'
miRNA:   3'- aaGUACUaCAu-----UGGUACGGUUG-CGGG- -5'
12282 3' -49.7 NC_003313.1 + 26007 0.7 0.59618
Target:  5'- -gCGUGAcguUGUAuucCCAUGCUuuuagcucuAGCGCCCa -3'
miRNA:   3'- aaGUACU---ACAUu--GGUACGG---------UUGCGGG- -5'
12282 3' -49.7 NC_003313.1 + 10000 1.11 0.001298
Target:  5'- aUUCAUGAUGUAACCAUGCCAACGCCCu -3'
miRNA:   3'- -AAGUACUACAUUGGUACGGUUGCGGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.