Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12282 | 5' | -55.9 | NC_003313.1 | + | 24909 | 0.68 | 0.403978 |
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Target: 5'- cCAcAGCGCGGCGuugaauaaccaugUGGCGGCgguCAUGGc -3' miRNA: 3'- -GUcUCGCGUCGU-------------GUCGCCGau-GUACC- -5' |
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| 12282 | 5' | -55.9 | NC_003313.1 | + | 30770 | 0.69 | 0.349766 |
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Target: 5'- gCAG-GUGCuuGGCGCGGCGGCUuguucCAauUGGg -3' miRNA: 3'- -GUCuCGCG--UCGUGUCGCCGAu----GU--ACC- -5' |
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| 12282 | 5' | -55.9 | NC_003313.1 | + | 8281 | 0.71 | 0.256161 |
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Target: 5'- aAGGGCGCAcGCGCGGCGaauagcuuuCUACGUGa -3' miRNA: 3'- gUCUCGCGU-CGUGUCGCc--------GAUGUACc -5' |
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| 12282 | 5' | -55.9 | NC_003313.1 | + | 4986 | 0.71 | 0.236217 |
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Target: 5'- -uGuGCGCggcgGGCACAuGCGGCaGCGUGGc -3' miRNA: 3'- guCuCGCG----UCGUGU-CGCCGaUGUACC- -5' |
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| 12282 | 5' | -55.9 | NC_003313.1 | + | 9966 | 1.04 | 0.000889 |
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Target: 5'- gCAGAGCGCAGCACAGCGGCUACAUGu -3' miRNA: 3'- -GUCUCGCGUCGUGUCGCCGAUGUACc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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