Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12285 | 3' | -58.8 | NC_003313.1 | + | 8682 | 0.66 | 0.354781 |
Target: 5'- --cGCGGuCGGCGUGuuugcGGCUaGCagugaGCUGCa -3' miRNA: 3'- cguCGUC-GCCGUAC-----UCGA-CGg----CGACG- -5' |
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12285 | 3' | -58.8 | NC_003313.1 | + | 5516 | 0.66 | 0.346148 |
Target: 5'- -uGGCGGCaaGCAUGAGaaGCCGCgaUGUu -3' miRNA: 3'- cgUCGUCGc-CGUACUCgaCGGCG--ACG- -5' |
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12285 | 3' | -58.8 | NC_003313.1 | + | 11515 | 0.67 | 0.305269 |
Target: 5'- uCAGCAGCaGGUugccGAGCgUGCuggcguuuCGCUGCg -3' miRNA: 3'- cGUCGUCG-CCGua--CUCG-ACG--------GCGACG- -5' |
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12285 | 3' | -58.8 | NC_003313.1 | + | 14413 | 0.68 | 0.271024 |
Target: 5'- -aAGCAGUGGCGuuaugugguuacgauUGAGgaUGCCGCUa- -3' miRNA: 3'- cgUCGUCGCCGU---------------ACUCg-ACGGCGAcg -5' |
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12285 | 3' | -58.8 | NC_003313.1 | + | 4472 | 0.68 | 0.261233 |
Target: 5'- uCAGUgauuGCGGCGU-AGUUGCCGUgGCg -3' miRNA: 3'- cGUCGu---CGCCGUAcUCGACGGCGaCG- -5' |
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12285 | 3' | -58.8 | NC_003313.1 | + | 28416 | 0.68 | 0.254417 |
Target: 5'- uUAGCAGUGGCuUGcGCUauaucGCCcgcGCUGCg -3' miRNA: 3'- cGUCGUCGCCGuACuCGA-----CGG---CGACG- -5' |
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12285 | 3' | -58.8 | NC_003313.1 | + | 32407 | 0.7 | 0.177089 |
Target: 5'- aGCGGCAucaauGCGGCGgcuuacuucuucgcGUUGCUGCUGCa -3' miRNA: 3'- -CGUCGU-----CGCCGUacu-----------CGACGGCGACG- -5' |
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12285 | 3' | -58.8 | NC_003313.1 | + | 27398 | 0.71 | 0.16419 |
Target: 5'- aUAGCGGCGGCAgGuuCUGCCGUauUGUu -3' miRNA: 3'- cGUCGUCGCCGUaCucGACGGCG--ACG- -5' |
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12285 | 3' | -58.8 | NC_003313.1 | + | 12571 | 0.73 | 0.119979 |
Target: 5'- aGCGGCucggcaaauuGGCGGCuuuauuacUGAGCacggugGCCGCUGUg -3' miRNA: 3'- -CGUCG----------UCGCCGu-------ACUCGa-----CGGCGACG- -5' |
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12285 | 3' | -58.8 | NC_003313.1 | + | 17599 | 0.82 | 0.021187 |
Target: 5'- cCAGCAGCGGCAgcucaUGCCGCUGCu -3' miRNA: 3'- cGUCGUCGCCGUacucgACGGCGACG- -5' |
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12285 | 3' | -58.8 | NC_003313.1 | + | 17660 | 1.13 | 8.6e-05 |
Target: 5'- cGCAGCAGCGGCAUGAGCUGCCGCUGCu -3' miRNA: 3'- -CGUCGUCGCCGUACUCGACGGCGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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