Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12287 | 3' | -51.5 | NC_003313.1 | + | 25502 | 0.66 | 0.766449 |
Target: 5'- uGCUUAauGUCCUGCGCGaugcuguaaaggucGCUGCAUuggguuGGCu -3' miRNA: 3'- -CGAGU--UAGGACGUGU--------------CGGCGUGu-----UUG- -5' |
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12287 | 3' | -51.5 | NC_003313.1 | + | 4874 | 0.66 | 0.747559 |
Target: 5'- uGCcgCAGUaCCacGCaccacGCAGCCGCGCAGAa -3' miRNA: 3'- -CGa-GUUA-GGa-CG-----UGUCGGCGUGUUUg -5' |
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12287 | 3' | -51.5 | NC_003313.1 | + | 17914 | 0.66 | 0.724834 |
Target: 5'- cGCUCAAg-CUGgGCAGUuauuUGCGCGGAUg -3' miRNA: 3'- -CGAGUUagGACgUGUCG----GCGUGUUUG- -5' |
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12287 | 3' | -51.5 | NC_003313.1 | + | 22824 | 0.66 | 0.708664 |
Target: 5'- --aCAuUCCUGaugcuguuacucaGCGGCUGCGCGAACc -3' miRNA: 3'- cgaGUuAGGACg------------UGUCGGCGUGUUUG- -5' |
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12287 | 3' | -51.5 | NC_003313.1 | + | 22897 | 0.68 | 0.606987 |
Target: 5'- aGUaCGGUUC-GCGCAGCCGCugAguAACa -3' miRNA: 3'- -CGaGUUAGGaCGUGUCGGCGugU--UUG- -5' |
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12287 | 3' | -51.5 | NC_003313.1 | + | 4443 | 0.7 | 0.513767 |
Target: 5'- gGCUCAGU-UUGUGCGGCUGUGCAGGa -3' miRNA: 3'- -CGAGUUAgGACGUGUCGGCGUGUUUg -5' |
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12287 | 3' | -51.5 | NC_003313.1 | + | 18453 | 0.72 | 0.396656 |
Target: 5'- uUUCGAacgCCUGCGuggaacgcCAGCCGCGCAAGa -3' miRNA: 3'- cGAGUUa--GGACGU--------GUCGGCGUGUUUg -5' |
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12287 | 3' | -51.5 | NC_003313.1 | + | 18529 | 0.78 | 0.169275 |
Target: 5'- cGCUCAucGUCUUGCGCGGCUGgcguuccaCGCAGGCg -3' miRNA: 3'- -CGAGU--UAGGACGUGUCGGC--------GUGUUUG- -5' |
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12287 | 3' | -51.5 | NC_003313.1 | + | 4379 | 1.12 | 0.000647 |
Target: 5'- uGCUCAAUCCUGCACAGCCGCACAAACu -3' miRNA: 3'- -CGAGUUAGGACGUGUCGGCGUGUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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