Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12288 | 5' | -50.6 | NC_003313.1 | + | 20868 | 0.66 | 0.843668 |
Target: 5'- uCGCCG-CuGCAAagcucuauuucACCCAAGAuuugCGCa -3' miRNA: 3'- -GCGGUaGuCGUU-----------UGGGUUCUuca-GCG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 29945 | 0.66 | 0.840859 |
Target: 5'- gGCCAgcgUCAGCAGGCUCAauccagaacacgguGGAaucAGUCa- -3' miRNA: 3'- gCGGU---AGUCGUUUGGGU--------------UCU---UCAGcg -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 10326 | 0.66 | 0.824521 |
Target: 5'- uCGCCAUCAGC--GCCCuguuuaAAGgcaGCa -3' miRNA: 3'- -GCGGUAGUCGuuUGGGuuc---UUCag-CG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 24211 | 0.66 | 0.814592 |
Target: 5'- uCGCCAUCuguucugacauGGCGAuACCUgcuGcGGUCGCg -3' miRNA: 3'- -GCGGUAG-----------UCGUU-UGGGuu-CuUCAGCG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 7406 | 0.66 | 0.804441 |
Target: 5'- uCGCCA--AGCAagaaAACCagaAGGAGGcCGCa -3' miRNA: 3'- -GCGGUagUCGU----UUGGg--UUCUUCaGCG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 19642 | 0.66 | 0.794082 |
Target: 5'- cCGCCAgaaagCGGCAAG-CCAAGcagcaaaauGAGcCGCg -3' miRNA: 3'- -GCGGUa----GUCGUUUgGGUUC---------UUCaGCG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 31101 | 0.67 | 0.783528 |
Target: 5'- cCGCCGUaCAGCAAGaaaaCAAGAuggcGUgGCa -3' miRNA: 3'- -GCGGUA-GUCGUUUgg--GUUCUu---CAgCG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 32828 | 0.67 | 0.750833 |
Target: 5'- cCGCCAUCAcGCc-GCCguGGGuaugGGUUGCg -3' miRNA: 3'- -GCGGUAGU-CGuuUGGguUCU----UCAGCG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 25106 | 0.67 | 0.73851 |
Target: 5'- cCGCCAUC-GCAAAagCCGuuccuguacugccAGAuGUCGCg -3' miRNA: 3'- -GCGGUAGuCGUUUg-GGU-------------UCUuCAGCG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 4715 | 0.68 | 0.705355 |
Target: 5'- gGCgAUCAGUAAugCCA---AGUUGCc -3' miRNA: 3'- gCGgUAGUCGUUugGGUucuUCAGCG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 10051 | 0.7 | 0.57602 |
Target: 5'- aGCCAUCcGCAAGCU--GGAAGgcaUCGCc -3' miRNA: 3'- gCGGUAGuCGUUUGGguUCUUC---AGCG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 24869 | 0.72 | 0.451516 |
Target: 5'- gCGCUGUgGGCAAACCCAaucacauGGAuuaUCGCg -3' miRNA: 3'- -GCGGUAgUCGUUUGGGU-------UCUuc-AGCG- -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 11976 | 0.73 | 0.421402 |
Target: 5'- aGCCAuucUCAGCAuuauugagauGGCCUcAGAAGUCGa -3' miRNA: 3'- gCGGU---AGUCGU----------UUGGGuUCUUCAGCg -5' |
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12288 | 5' | -50.6 | NC_003313.1 | + | 6537 | 1.12 | 0.000942 |
Target: 5'- cCGCCAUCAGCAAACCCAAGAAGUCGCc -3' miRNA: 3'- -GCGGUAGUCGUUUGGGUUCUUCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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