Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1229 | 5' | -45.1 | NC_001271.1 | + | 33120 | 0.66 | 0.992273 |
Target: 5'- -aCGAAUGGUGAACUUAC--CGUCUUc -3' miRNA: 3'- uaGCUUAUUAUUUGGAUGucGCAGGA- -5' |
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1229 | 5' | -45.1 | NC_001271.1 | + | 38511 | 0.66 | 0.990936 |
Target: 5'- cAUCGAGUcggucaucAUGGGCC-ACAGCGcCCUu -3' miRNA: 3'- -UAGCUUAu-------UAUUUGGaUGUCGCaGGA- -5' |
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1229 | 5' | -45.1 | NC_001271.1 | + | 29734 | 0.67 | 0.981326 |
Target: 5'- gGUCGAAccgAGUgcGAACCUGCccgguuGUGUCCa -3' miRNA: 3'- -UAGCUUa--UUA--UUUGGAUGu-----CGCAGGa -5' |
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1229 | 5' | -45.1 | NC_001271.1 | + | 25987 | 0.67 | 0.97872 |
Target: 5'- -aCGGAUGGUGAACgCUcCAGaGUCCUc -3' miRNA: 3'- uaGCUUAUUAUUUG-GAuGUCgCAGGA- -5' |
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1229 | 5' | -45.1 | NC_001271.1 | + | 17842 | 0.68 | 0.96929 |
Target: 5'- -gCGAugGUAAUcAGCUUcGCGGCGUCCa -3' miRNA: 3'- uaGCU--UAUUAuUUGGA-UGUCGCAGGa -5' |
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1229 | 5' | -45.1 | NC_001271.1 | + | 29479 | 0.73 | 0.812045 |
Target: 5'- cGUUG-AUGGUGAuACCUAUGGUGUCCUg -3' miRNA: 3'- -UAGCuUAUUAUU-UGGAUGUCGCAGGA- -5' |
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1229 | 5' | -45.1 | NC_001271.1 | + | 28386 | 0.74 | 0.73375 |
Target: 5'- -cUGAAUGAc-GugCUACAGCGUCCg -3' miRNA: 3'- uaGCUUAUUauUugGAUGUCGCAGGa -5' |
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1229 | 5' | -45.1 | NC_001271.1 | + | 12300 | 1.06 | 0.010771 |
Target: 5'- cAUCGAAUAAUAAACCUACAGCGUCCUc -3' miRNA: 3'- -UAGCUUAUUAUUUGGAUGUCGCAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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