Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12291 | 3' | -55.2 | NC_003313.1 | + | 31230 | 0.66 | 0.563955 |
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Target: 5'- -aGCUCGCCCGCg-CUGCucuuucuGCUCGGcuACa -3' miRNA: 3'- cgCGAGCGGGUGaaGACG-------UGGGUU--UG- -5' |
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| 12291 | 3' | -55.2 | NC_003313.1 | + | 28428 | 0.66 | 0.553775 |
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Target: 5'- uGCGCuauaUCGCCCGCg-CUGCgucagGgCCAGAUa -3' miRNA: 3'- -CGCG----AGCGGGUGaaGACG-----UgGGUUUG- -5' |
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| 12291 | 3' | -55.2 | NC_003313.1 | + | 19852 | 0.67 | 0.466355 |
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Target: 5'- cCGCuUCGCCCACUgucauguuuUCUcGUAUCCAuGCc -3' miRNA: 3'- cGCG-AGCGGGUGA---------AGA-CGUGGGUuUG- -5' |
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| 12291 | 3' | -55.2 | NC_003313.1 | + | 16916 | 0.67 | 0.466355 |
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Target: 5'- -gGUUCGCCCACUg--GCagagcauuGCCCAAGu -3' miRNA: 3'- cgCGAGCGGGUGAagaCG--------UGGGUUUg -5' |
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| 12291 | 3' | -55.2 | NC_003313.1 | + | 25146 | 0.67 | 0.455916 |
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Target: 5'- gGCGCUUGaa-GCcUC-GCGCCCAAGCa -3' miRNA: 3'- -CGCGAGCgggUGaAGaCGUGGGUUUG- -5' |
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| 12291 | 3' | -55.2 | NC_003313.1 | + | 27051 | 0.7 | 0.324649 |
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Target: 5'- cGCGCUCaCCUGCagugaaaaaaUUCUGCGCCUGAugGCg -3' miRNA: 3'- -CGCGAGcGGGUG----------AAGACGUGGGUU--UG- -5' |
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| 12291 | 3' | -55.2 | NC_003313.1 | + | 4965 | 0.71 | 0.300443 |
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Target: 5'- gGCGCaCGCCCugUUUgaaaGCAgCUCAAGCg -3' miRNA: 3'- -CGCGaGCGGGugAAGa---CGU-GGGUUUG- -5' |
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| 12291 | 3' | -55.2 | NC_003313.1 | + | 8688 | 1.11 | 0.00032 |
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Target: 5'- gGCGCUCGCCCACUUCUGCACCCAAACu -3' miRNA: 3'- -CGCGAGCGGGUGAAGACGUGGGUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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