Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12291 | 5' | -47 | NC_003313.1 | + | 1757 | 0.66 | 0.962507 |
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Target: 5'- aCC-CGCAAGgccgCGAAGGUAAGCgCGu -3' miRNA: 3'- -GGcGCGUUCaua-GUUUUCGUUUGgGU- -5' |
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| 12291 | 5' | -47 | NC_003313.1 | + | 9827 | 0.66 | 0.953747 |
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Target: 5'- gCCaGCGCGAGUcaugaccaAUCAagaacugguuGAAGUGAucGCCCAa -3' miRNA: 3'- -GG-CGCGUUCA--------UAGU----------UUUCGUU--UGGGU- -5' |
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| 12291 | 5' | -47 | NC_003313.1 | + | 30143 | 0.68 | 0.912748 |
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Target: 5'- aCCGCGCAGGUu----GAGUAAcGCCUc -3' miRNA: 3'- -GGCGCGUUCAuaguuUUCGUU-UGGGu -5' |
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| 12291 | 5' | -47 | NC_003313.1 | + | 12779 | 0.68 | 0.881992 |
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Target: 5'- aCCGCaGCAAGgugCGAAuguGCcGGACCCGc -3' miRNA: 3'- -GGCG-CGUUCauaGUUUu--CG-UUUGGGU- -5' |
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| 12291 | 5' | -47 | NC_003313.1 | + | 4945 | 0.69 | 0.855624 |
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Target: 5'- gCCGCGCAcAGUAUugCGAuGGCGcacGCCCu -3' miRNA: 3'- -GGCGCGU-UCAUA--GUUuUCGUu--UGGGu -5' |
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| 12291 | 5' | -47 | NC_003313.1 | + | 25898 | 0.69 | 0.845298 |
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Target: 5'- ---aGCAGGUGUCAcaacggcAgcGCGAACCCAa -3' miRNA: 3'- ggcgCGUUCAUAGU-------UuuCGUUUGGGU- -5' |
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| 12291 | 5' | -47 | NC_003313.1 | + | 14403 | 0.71 | 0.773677 |
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Target: 5'- aUCGCGC-AGUucAUCAAAGGUcggGAACUCAa -3' miRNA: 3'- -GGCGCGuUCA--UAGUUUUCG---UUUGGGU- -5' |
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| 12291 | 5' | -47 | NC_003313.1 | + | 24866 | 0.72 | 0.69241 |
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Target: 5'- gCCGCGCu-GUG-----GGCAAACCCAa -3' miRNA: 3'- -GGCGCGuuCAUaguuuUCGUUUGGGU- -5' |
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| 12291 | 5' | -47 | NC_003313.1 | + | 22280 | 0.81 | 0.247801 |
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Target: 5'- gCUGCGUggaAAGUGUCGAAcgcgccucaGGCAAACCCAu -3' miRNA: 3'- -GGCGCG---UUCAUAGUUU---------UCGUUUGGGU- -5' |
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| 12291 | 5' | -47 | NC_003313.1 | + | 8730 | 1.12 | 0.002523 |
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Target: 5'- gCCGCGCAAGUAUCAAAAGCAAACCCAc -3' miRNA: 3'- -GGCGCGUUCAUAGUUUUCGUUUGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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