Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12293 | 3' | -51.5 | NC_003313.1 | + | 18567 | 0.66 | 0.744235 |
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Target: 5'- --cAUCGCUGCAGgcCAUgccaGCGCAGCa-- -3' miRNA: 3'- gauUGGCGACGUU--GUA----UGCGUCGcuu -5' |
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| 12293 | 3' | -51.5 | NC_003313.1 | + | 4935 | 0.66 | 0.732828 |
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Target: 5'- --cGCgGCUGCGugGUGCGUGGUa-- -3' miRNA: 3'- gauUGgCGACGUugUAUGCGUCGcuu -5' |
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| 12293 | 3' | -51.5 | NC_003313.1 | + | 4432 | 0.66 | 0.70967 |
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Target: 5'- --uGCgGCUGUgcaggauugAGCAUguuGCGCAGCGAu -3' miRNA: 3'- gauUGgCGACG---------UUGUA---UGCGUCGCUu -5' |
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| 12293 | 3' | -51.5 | NC_003313.1 | + | 18628 | 0.67 | 0.650455 |
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Target: 5'- ---uCUGCUGCGcugGCAUGgccUGCAGCGAu -3' miRNA: 3'- gauuGGCGACGU---UGUAU---GCGUCGCUu -5' |
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| 12293 | 3' | -51.5 | NC_003313.1 | + | 30073 | 0.68 | 0.595483 |
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Target: 5'- uUAAgCGCUGCAGCaGaggcguuacucaaccUGCGCGGUGAGu -3' miRNA: 3'- gAUUgGCGACGUUG-U---------------AUGCGUCGCUU- -5' |
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| 12293 | 3' | -51.5 | NC_003313.1 | + | 12533 | 0.69 | 0.555281 |
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Target: 5'- gUGGCCGCUGUGugGUGgcggguuuUGCGGUGAu -3' miRNA: 3'- gAUUGGCGACGUugUAU--------GCGUCGCUu -5' |
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| 12293 | 3' | -51.5 | NC_003313.1 | + | 4988 | 0.69 | 0.543598 |
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Target: 5'- aCUGugCGCgGCGggcACAUGCGgCAGCGu- -3' miRNA: 3'- -GAUugGCGaCGU---UGUAUGC-GUCGCuu -5' |
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| 12293 | 3' | -51.5 | NC_003313.1 | + | 11218 | 0.71 | 0.443079 |
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Target: 5'- gCUAugCGCUGCGGCuuUGCGCcGCa-- -3' miRNA: 3'- -GAUugGCGACGUUGu-AUGCGuCGcuu -5' |
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| 12293 | 3' | -51.5 | NC_003313.1 | + | 11427 | 1.06 | 0.001473 |
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Target: 5'- uCUAACCGCUGCAACAUACGCAGCGAAa -3' miRNA: 3'- -GAUUGGCGACGUUGUAUGCGUCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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