Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12294 | 3' | -45.4 | NC_003313.1 | + | 18791 | 0.66 | 0.987192 |
Target: 5'- gCAAUAugCUCGGcaagaaUCGCgCAAGcGUGGa -3' miRNA: 3'- -GUUAUugGAGUC------AGCG-GUUUaUACCg -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 20120 | 0.66 | 0.987192 |
Target: 5'- --uUAGCCgaagcCAG-CGCCGAAg--GGCu -3' miRNA: 3'- guuAUUGGa----GUCaGCGGUUUauaCCG- -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 6375 | 0.66 | 0.983013 |
Target: 5'- cCAGcGGCCU---UCGCCAucggcAUGUGGCa -3' miRNA: 3'- -GUUaUUGGAgucAGCGGUu----UAUACCG- -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 5384 | 0.67 | 0.971682 |
Target: 5'- aAGUcACaCUCAGUaaccaaCGCCAAGUGacauUGGCc -3' miRNA: 3'- gUUAuUG-GAGUCA------GCGGUUUAU----ACCG- -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 20432 | 0.68 | 0.960183 |
Target: 5'- --uUAGCCgacaCGGUCGacaucCCAaaaGAUGUGGCg -3' miRNA: 3'- guuAUUGGa---GUCAGC-----GGU---UUAUACCG- -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 11650 | 0.68 | 0.955722 |
Target: 5'- --cUAACCUCAGUUGgCAuaac-GGCu -3' miRNA: 3'- guuAUUGGAGUCAGCgGUuuauaCCG- -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 8295 | 0.69 | 0.934543 |
Target: 5'- -uGUAGCgUUCAGcaaaguauuUCGCgAGGUAUGGCg -3' miRNA: 3'- guUAUUG-GAGUC---------AGCGgUUUAUACCG- -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 22227 | 0.69 | 0.915049 |
Target: 5'- ---aAGCCUUgagcaAGUCGCCAAAgauUGGg -3' miRNA: 3'- guuaUUGGAG-----UCAGCGGUUUau-ACCg -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 31017 | 0.69 | 0.915049 |
Target: 5'- gCGGUGAUCgcaagguuuaUCgAGUCGCUAacGAUGUGGCc -3' miRNA: 3'- -GUUAUUGG----------AG-UCAGCGGU--UUAUACCG- -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 1579 | 0.7 | 0.892465 |
Target: 5'- gCAAUucaCUCAGUCGCCuauUAUcaGGCu -3' miRNA: 3'- -GUUAuugGAGUCAGCGGuuuAUA--CCG- -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 21894 | 0.72 | 0.818359 |
Target: 5'- ----uGCCcUAGUCGCCAAuauuUGGCa -3' miRNA: 3'- guuauUGGaGUCAGCGGUUuau-ACCG- -5' |
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12294 | 3' | -45.4 | NC_003313.1 | + | 11558 | 1.14 | 0.002565 |
Target: 5'- uCAAUAACCUCAGUCGCCAAAUAUGGCg -3' miRNA: 3'- -GUUAUUGGAGUCAGCGGUUUAUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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