Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12296 | 3' | -59.3 | NC_003313.1 | + | 14384 | 0.66 | 0.350001 |
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Target: 5'- cUGGacacaaGCGACCGCCAUC-GCGCaGUu -3' miRNA: 3'- cACCg-----CGCUGGUGGUAGuCGUGgCGu -5' |
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| 12296 | 3' | -59.3 | NC_003313.1 | + | 9345 | 0.67 | 0.272014 |
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Target: 5'- uGUGGCGUG-CCAUCGugaugcggcUCGGCAacUCGCAc -3' miRNA: 3'- -CACCGCGCuGGUGGU---------AGUCGU--GGCGU- -5' |
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| 12296 | 3' | -59.3 | NC_003313.1 | + | 32842 | 0.68 | 0.244962 |
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Target: 5'- gGUGGCuGCaGGucCCGCCAUCA-CGCCGCc -3' miRNA: 3'- -CACCG-CG-CU--GGUGGUAGUcGUGGCGu -5' |
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| 12296 | 3' | -59.3 | NC_003313.1 | + | 32790 | 0.72 | 0.133743 |
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Target: 5'- aUGGCGgGACCugCAgccaccgCAGCACCa-- -3' miRNA: 3'- cACCGCgCUGGugGUa------GUCGUGGcgu -5' |
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| 12296 | 3' | -59.3 | NC_003313.1 | + | 8905 | 0.73 | 0.11596 |
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Target: 5'- uGUGuGCGCGAgCACCAagagCAGCAacaaCGCAa -3' miRNA: 3'- -CAC-CGCGCUgGUGGUa---GUCGUg---GCGU- -5' |
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| 12296 | 3' | -59.3 | NC_003313.1 | + | 14077 | 1.07 | 0.000229 |
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Target: 5'- gGUGGCGCGACCACCAUCAGCACCGCAu -3' miRNA: 3'- -CACCGCGCUGGUGGUAGUCGUGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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