Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12297 | 3' | -50.5 | NC_003313.1 | + | 29122 | 0.66 | 0.77545 |
Target: 5'- uCAGCAGCGCaaaGCCcUAucAAUgGGCAGa- -3' miRNA: 3'- -GUCGUCGUG---CGGuAU--UUAaCCGUCau -5' |
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12297 | 3' | -50.5 | NC_003313.1 | + | 6375 | 0.66 | 0.77545 |
Target: 5'- cCAGCGGCcuuCGCCAUcggcaugUGGCAa-- -3' miRNA: 3'- -GUCGUCGu--GCGGUAuuua---ACCGUcau -5' |
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12297 | 3' | -50.5 | NC_003313.1 | + | 12536 | 0.66 | 0.752962 |
Target: 5'- aCGGUGGC-CGCUGUGuggUGGCGGg- -3' miRNA: 3'- -GUCGUCGuGCGGUAUuuaACCGUCau -5' |
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12297 | 3' | -50.5 | NC_003313.1 | + | 14125 | 0.67 | 0.70631 |
Target: 5'- uGGUGGuCGCGCCAccGAUugUGGCGGUu -3' miRNA: 3'- gUCGUC-GUGCGGUauUUA--ACCGUCAu -5' |
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12297 | 3' | -50.5 | NC_003313.1 | + | 13400 | 0.68 | 0.670338 |
Target: 5'- uGGCGGgGCGUuuUAUGAA-UGGCGGUGg -3' miRNA: 3'- gUCGUCgUGCG--GUAUUUaACCGUCAU- -5' |
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12297 | 3' | -50.5 | NC_003313.1 | + | 9101 | 0.69 | 0.561513 |
Target: 5'- aCGGCAGCGCaCCAUcAAUucaUGGCGGc- -3' miRNA: 3'- -GUCGUCGUGcGGUAuUUA---ACCGUCau -5' |
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12297 | 3' | -50.5 | NC_003313.1 | + | 652 | 0.7 | 0.549614 |
Target: 5'- uGGCGGUggguuuguCGCCAUAAAU-GGCGGUu -3' miRNA: 3'- gUCGUCGu-------GCGGUAUUUAaCCGUCAu -5' |
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12297 | 3' | -50.5 | NC_003313.1 | + | 17563 | 1.07 | 0.001554 |
Target: 5'- cCAGCAGCACGCCAUAAAUUGGCAGUAu -3' miRNA: 3'- -GUCGUCGUGCGGUAUUUAACCGUCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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