Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12297 | 5' | -60.2 | NC_003313.1 | + | 22902 | 0.66 | 0.275309 |
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Target: 5'- -cCGGCGGgAauGCUCGUGCC-CUGCa- -3' miRNA: 3'- ucGUCGCCgU--CGAGUACGGcGACGac -5' |
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| 12297 | 5' | -60.2 | NC_003313.1 | + | 11445 | 0.67 | 0.247748 |
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Target: 5'- cGCAGCGaaacgccaGCAcGCUCGgcaacCUGCUGCUGa -3' miRNA: 3'- uCGUCGC--------CGU-CGAGUac---GGCGACGAC- -5' |
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| 12297 | 5' | -60.2 | NC_003313.1 | + | 20910 | 0.69 | 0.178576 |
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Target: 5'- uGCAGCGGCGauGCUC-UGCgGC-GUUGa -3' miRNA: 3'- uCGUCGCCGU--CGAGuACGgCGaCGAC- -5' |
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| 12297 | 5' | -60.2 | NC_003313.1 | + | 27397 | 0.71 | 0.119979 |
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Target: 5'- uAGCGGCGGCAGgUUcUGCCGUauUGUUc -3' miRNA: 3'- -UCGUCGCCGUCgAGuACGGCG--ACGAc -5' |
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| 12297 | 5' | -60.2 | NC_003313.1 | + | 30062 | 0.72 | 0.097892 |
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Target: 5'- cGGcCAGUGGCAGCacUCAUGgUGCUGCc- -3' miRNA: 3'- -UC-GUCGCCGUCG--AGUACgGCGACGac -5' |
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| 12297 | 5' | -60.2 | NC_003313.1 | + | 32405 | 0.74 | 0.075109 |
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Target: 5'- cGGCAucaauGCGGCGGCUUAcuucuucgcguUGCUGCUGCa- -3' miRNA: 3'- -UCGU-----CGCCGUCGAGU-----------ACGGCGACGac -5' |
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| 12297 | 5' | -60.2 | NC_003313.1 | + | 17658 | 0.81 | 0.01819 |
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Target: 5'- cAGCAGCGGCaugAGC---UGCCGCUGCUGg -3' miRNA: 3'- -UCGUCGCCG---UCGaguACGGCGACGAC- -5' |
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| 12297 | 5' | -60.2 | NC_003313.1 | + | 17600 | 1.07 | 0.000165 |
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Target: 5'- cAGCAGCGGCAGCUCAUGCCGCUGCUGc -3' miRNA: 3'- -UCGUCGCCGUCGAGUACGGCGACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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