Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12298 | 5' | -53.8 | NC_003313.1 | + | 14327 | 0.66 | 0.625502 |
Target: 5'- gGUCAcAGCCaCCC---UUAGCGGCAu -3' miRNA: 3'- aCAGUuUCGGcGGGucgAAUUGCCGU- -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 17586 | 0.66 | 0.625502 |
Target: 5'- aGUauuAGCCacCCCAGC--AGCGGCAg -3' miRNA: 3'- aCAguuUCGGc-GGGUCGaaUUGCCGU- -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 16906 | 0.66 | 0.625502 |
Target: 5'- cUGgCAGAGCauuGCCCaAGUgaUGGCGGCAc -3' miRNA: 3'- -ACaGUUUCGg--CGGG-UCGa-AUUGCCGU- -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 14604 | 0.67 | 0.544321 |
Target: 5'- --aCAAAGUCGCCuCGGC--GAUGGCu -3' miRNA: 3'- acaGUUUCGGCGG-GUCGaaUUGCCGu -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 12579 | 0.67 | 0.544321 |
Target: 5'- aGUgaUGAAGCgGCUCGGCaaaUUGGCGGCu -3' miRNA: 3'- aCA--GUUUCGgCGGGUCG---AAUUGCCGu -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 21350 | 0.68 | 0.48192 |
Target: 5'- cGUCAGguuugaucccgcgauAGCUGCCC-GCUcgacgGGCGGCu -3' miRNA: 3'- aCAGUU---------------UCGGCGGGuCGAa----UUGCCGu -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 17674 | 0.69 | 0.445762 |
Target: 5'- cUGUCGAGGCUuuCCgCAGC--AGCGGCAu -3' miRNA: 3'- -ACAGUUUCGGc-GG-GUCGaaUUGCCGU- -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 9206 | 0.69 | 0.440572 |
Target: 5'- gGUCGAuaaAGCCGCCUuuuuuguAGCUguugugguacuucGACGGCGu -3' miRNA: 3'- aCAGUU---UCGGCGGG-------UCGAa------------UUGCCGU- -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 955 | 0.69 | 0.425213 |
Target: 5'- gGUC-AAGCCaCCCAcGUUUuACGGCAu -3' miRNA: 3'- aCAGuUUCGGcGGGU-CGAAuUGCCGU- -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 21271 | 0.7 | 0.367117 |
Target: 5'- --aCAAAGCCGCCC-GUcgAGCgGGCAg -3' miRNA: 3'- acaGUUUCGGCGGGuCGaaUUG-CCGU- -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 22547 | 0.72 | 0.290717 |
Target: 5'- -aUUAAAGCCa-UCGGCUUAGCGGCAu -3' miRNA: 3'- acAGUUUCGGcgGGUCGAAUUGCCGU- -5' |
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12298 | 5' | -53.8 | NC_003313.1 | + | 20151 | 1.09 | 0.000571 |
Target: 5'- uUGUCAAAGCCGCCCAGCUUAACGGCAa -3' miRNA: 3'- -ACAGUUUCGGCGGGUCGAAUUGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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