Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12299 | 3' | -55.3 | NC_003313.1 | + | 4862 | 0.66 | 0.549337 |
Target: 5'- -aUUGCGCugCAcuugcUGGCGCguuucugCGauGGCu -3' miRNA: 3'- uaAACGCGugGU-----ACCGUGa------GUgcCCG- -5' |
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12299 | 3' | -55.3 | NC_003313.1 | + | 16609 | 0.66 | 0.526854 |
Target: 5'- ---aGCGaaguuauCCAU-GUAUUCACGGGCg -3' miRNA: 3'- uaaaCGCgu-----GGUAcCGUGAGUGCCCG- -5' |
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12299 | 3' | -55.3 | NC_003313.1 | + | 9096 | 0.66 | 0.504724 |
Target: 5'- ---aGCGCACCAucaauucaUGGCGgcgaaUCGCaGGGUu -3' miRNA: 3'- uaaaCGCGUGGU--------ACCGUg----AGUG-CCCG- -5' |
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12299 | 3' | -55.3 | NC_003313.1 | + | 24740 | 0.66 | 0.503628 |
Target: 5'- ---aGCGCACCAauggcggcauaacUGaGCACUucgguuagCACGGGa -3' miRNA: 3'- uaaaCGCGUGGU-------------AC-CGUGA--------GUGCCCg -5' |
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12299 | 3' | -55.3 | NC_003313.1 | + | 9057 | 0.67 | 0.430813 |
Target: 5'- --gUGCGCuGCCGUGGCugUgaagaCAUcGGCa -3' miRNA: 3'- uaaACGCG-UGGUACCGugA-----GUGcCCG- -5' |
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12299 | 3' | -55.3 | NC_003313.1 | + | 5004 | 0.68 | 0.420775 |
Target: 5'- ---cGUGCGCCAucgcaauacUGuGCGCg-GCGGGCa -3' miRNA: 3'- uaaaCGCGUGGU---------AC-CGUGagUGCCCG- -5' |
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12299 | 3' | -55.3 | NC_003313.1 | + | 19756 | 0.68 | 0.420775 |
Target: 5'- ---cGCGCAgCGuuUGGCuuGCUCuCGGGUg -3' miRNA: 3'- uaaaCGCGUgGU--ACCG--UGAGuGCCCG- -5' |
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12299 | 3' | -55.3 | NC_003313.1 | + | 20864 | 0.69 | 0.37278 |
Target: 5'- --aUGCGgGCaAUGGCACgaauacgcacuUUGCGGGCg -3' miRNA: 3'- uaaACGCgUGgUACCGUG-----------AGUGCCCG- -5' |
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12299 | 3' | -55.3 | NC_003313.1 | + | 19661 | 0.72 | 0.2181 |
Target: 5'- -cUUGCGCgauACCGUGGUgauguucguaagcgACUCgaccACGGGCg -3' miRNA: 3'- uaAACGCG---UGGUACCG--------------UGAG----UGCCCG- -5' |
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12299 | 3' | -55.3 | NC_003313.1 | + | 20897 | 1.11 | 0.000312 |
Target: 5'- gAUUUGCGCACCAUGGCACUCACGGGCg -3' miRNA: 3'- -UAAACGCGUGGUACCGUGAGUGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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