Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
123 | 5' | -52.7 | AC_000006.1 | + | 6330 | 0.66 | 0.747678 |
Target: 5'- ---aGCAGAugCUcgUCAGGGGgguCCGCa -3' miRNA: 3'- gacgCGUUUugGAcaAGUCCCU---GGCG- -5' |
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123 | 5' | -52.7 | AC_000006.1 | + | 8800 | 0.66 | 0.747678 |
Target: 5'- -cGCGCGAcGCCga--CGGGGacguggucuacaGCCGCg -3' miRNA: 3'- gaCGCGUUuUGGacaaGUCCC------------UGGCG- -5' |
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123 | 5' | -52.7 | AC_000006.1 | + | 12409 | 0.66 | 0.725518 |
Target: 5'- -cGCGCAgguucuugaAAGCCUGgcUCAGGuucACCGUu -3' miRNA: 3'- gaCGCGU---------UUUGGACa-AGUCCc--UGGCG- -5' |
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123 | 5' | -52.7 | AC_000006.1 | + | 16863 | 0.68 | 0.592105 |
Target: 5'- cCUGCGCAugGCCcgccgccggcggcgGUUCAGG--CCGCu -3' miRNA: 3'- -GACGCGUuuUGGa-------------CAAGUCCcuGGCG- -5' |
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123 | 5' | -52.7 | AC_000006.1 | + | 4921 | 0.68 | 0.587493 |
Target: 5'- cCUGCa-AGAGCuccucucgCUGggCGGGGACCGCc -3' miRNA: 3'- -GACGcgUUUUG--------GACaaGUCCCUGGCG- -5' |
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123 | 5' | -52.7 | AC_000006.1 | + | 10864 | 0.68 | 0.575995 |
Target: 5'- aUGCGCGAcuGCCgGUUucgggcgggCAGGGAgCUGCg -3' miRNA: 3'- gACGCGUUu-UGGaCAA---------GUCCCU-GGCG- -5' |
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123 | 5' | -52.7 | AC_000006.1 | + | 15572 | 0.69 | 0.519456 |
Target: 5'- -gGCGCgGAAACgUGUaCuGGGugCGCg -3' miRNA: 3'- gaCGCG-UUUUGgACAaGuCCCugGCG- -5' |
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123 | 5' | -52.7 | AC_000006.1 | + | 23204 | 0.69 | 0.519456 |
Target: 5'- gUG-GCAAgGGCC---UCGGGGACCGCa -3' miRNA: 3'- gACgCGUU-UUGGacaAGUCCCUGGCG- -5' |
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123 | 5' | -52.7 | AC_000006.1 | + | 11564 | 0.71 | 0.434145 |
Target: 5'- cCUGCGCcGGGCgCUGguaGGGGgcGCCGCc -3' miRNA: 3'- -GACGCGuUUUG-GACaagUCCC--UGGCG- -5' |
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123 | 5' | -52.7 | AC_000006.1 | + | 10487 | 0.71 | 0.424052 |
Target: 5'- -cGCgGCAGAACCcgGUUCGcGGacGGCCGCg -3' miRNA: 3'- gaCG-CGUUUUGGa-CAAGU-CC--CUGGCG- -5' |
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123 | 5' | -52.7 | AC_000006.1 | + | 10816 | 1.13 | 0.000502 |
Target: 5'- cCUGCGCAAAACCUGUUCAGGGACCGCa -3' miRNA: 3'- -GACGCGUUUUGGACAAGUCCCUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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