Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1230 | 3' | -45.2 | NC_001271.1 | + | 28144 | 0.66 | 0.993309 |
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Target: 5'- uCAGcGAAcGACUUAGAGUGcUCCuguaGGCg -3' miRNA: 3'- cGUUaCUUuCUGAGUCUCAU-AGG----UCG- -5' |
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| 1230 | 3' | -45.2 | NC_001271.1 | + | 158 | 0.69 | 0.956867 |
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Target: 5'- aGUGAUGGAuGGCUUaaagaggccuuaaAGAGUGUCCuAGUg -3' miRNA: 3'- -CGUUACUUuCUGAG-------------UCUCAUAGG-UCG- -5' |
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| 1230 | 3' | -45.2 | NC_001271.1 | + | 39487 | 0.69 | 0.956867 |
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Target: 5'- aGUGAUGGAuGGCUUaaagaggccuuaaAGAGUGUCCuAGUg -3' miRNA: 3'- -CGUUACUUuCUGAG-------------UCUCAUAGG-UCG- -5' |
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| 1230 | 3' | -45.2 | NC_001271.1 | + | 31637 | 0.72 | 0.871688 |
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Target: 5'- uGCAAUGAAAGACcCuGAGUucUCCAc- -3' miRNA: 3'- -CGUUACUUUCUGaGuCUCAu-AGGUcg -5' |
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| 1230 | 3' | -45.2 | NC_001271.1 | + | 30352 | 0.77 | 0.629593 |
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Target: 5'- cGCAA-GggGGACUUGGAGgcGUUCGGCg -3' miRNA: 3'- -CGUUaCuuUCUGAGUCUCa-UAGGUCG- -5' |
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| 1230 | 3' | -45.2 | NC_001271.1 | + | 18184 | 1.18 | 0.002067 |
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Target: 5'- cGCAAUGAAAGACUCAGAGUAUCCAGCa -3' miRNA: 3'- -CGUUACUUUCUGAGUCUCAUAGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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