Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12300 | 5' | -57.7 | NC_003313.1 | + | 23702 | 0.66 | 0.405945 |
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Target: 5'- cAGCCUGUUccaUGCCUuuuucAGCgGCACUCAUa -3' miRNA: 3'- -UCGGAUGA---ACGGA-----UCGgCGUGGGUGa -5' |
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| 12300 | 5' | -57.7 | NC_003313.1 | + | 4914 | 0.66 | 0.368647 |
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Target: 5'- uGCCUACcucGCCacgcuGCCGCauguGCCCGCc -3' miRNA: 3'- uCGGAUGaa-CGGau---CGGCG----UGGGUGa -5' |
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| 12300 | 5' | -57.7 | NC_003313.1 | + | 16835 | 0.67 | 0.325434 |
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Target: 5'- cGCCcACUUGCUUGGUgaaaCGgcCACCCGCUu -3' miRNA: 3'- uCGGaUGAACGGAUCG----GC--GUGGGUGA- -5' |
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| 12300 | 5' | -57.7 | NC_003313.1 | + | 12520 | 0.68 | 0.301368 |
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Target: 5'- cGCCaAUUUGCCgAGCCGCuucaUCACUg -3' miRNA: 3'- uCGGaUGAACGGaUCGGCGug--GGUGA- -5' |
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| 12300 | 5' | -57.7 | NC_003313.1 | + | 8606 | 0.73 | 0.124206 |
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Target: 5'- aAGCCUAUUUGCagcucacugCUAGCCGCAaaCACg -3' miRNA: 3'- -UCGGAUGAACG---------GAUCGGCGUggGUGa -5' |
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| 12300 | 5' | -57.7 | NC_003313.1 | + | 21630 | 1.08 | 0.000273 |
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Target: 5'- cAGCCUACUUGCCUAGCCGCACCCACUg -3' miRNA: 3'- -UCGGAUGAACGGAUCGGCGUGGGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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