Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12305 | 3' | -52.8 | NC_003313.1 | + | 8617 | 0.66 | 0.640774 |
Target: 5'- cAGCucACUGCuAGCcGCAAacacgccgacCGCGAAGAa -3' miRNA: 3'- cUCGu-UGACG-UCGuCGUU----------GCGCUUCU- -5' |
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12305 | 3' | -52.8 | NC_003313.1 | + | 31153 | 0.67 | 0.605249 |
Target: 5'- aGAGCAcaacAgUGUAGCcgagcagaaagAGCAGCGCGGGcGAg -3' miRNA: 3'- -CUCGU----UgACGUCG-----------UCGUUGCGCUU-CU- -5' |
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12305 | 3' | -52.8 | NC_003313.1 | + | 22389 | 0.67 | 0.593445 |
Target: 5'- cAGCAACcGCA-CAGCAACGC--AGAu -3' miRNA: 3'- cUCGUUGaCGUcGUCGUUGCGcuUCU- -5' |
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12305 | 3' | -52.8 | NC_003313.1 | + | 20886 | 0.69 | 0.468209 |
Target: 5'- uGAGUugaGugUGCGGuCAGCAAUGCGGGc- -3' miRNA: 3'- -CUCG---UugACGUC-GUCGUUGCGCUUcu -5' |
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12305 | 3' | -52.8 | NC_003313.1 | + | 31227 | 0.7 | 0.42602 |
Target: 5'- cGGC-ACUGCAacaagcacuGCAGCAaaAUGCGGAGGc -3' miRNA: 3'- cUCGuUGACGU---------CGUCGU--UGCGCUUCU- -5' |
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12305 | 3' | -52.8 | NC_003313.1 | + | 20917 | 0.7 | 0.42602 |
Target: 5'- aGAGCu-UUGCAGCGGCGAUGCu---- -3' miRNA: 3'- -CUCGuuGACGUCGUCGUUGCGcuucu -5' |
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12305 | 3' | -52.8 | NC_003313.1 | + | 4977 | 0.83 | 0.060218 |
Target: 5'- cGGGCAcaUGCGGCAGCGugGCGAGGu -3' miRNA: 3'- -CUCGUugACGUCGUCGUugCGCUUCu -5' |
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12305 | 3' | -52.8 | NC_003313.1 | + | 32324 | 1.09 | 0.000683 |
Target: 5'- uGAGCAACUGCAGCAGCAACGCGAAGAa -3' miRNA: 3'- -CUCGUUGACGUCGUCGUUGCGCUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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