Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12305 | 5' | -47.7 | NC_003313.1 | + | 24693 | 0.67 | 0.876865 |
Target: 5'- cCGcCAUUGGUGCgcuauCGCUgggcGGUGugGUUGCc -3' miRNA: 3'- -GC-GUAACUACG-----GCGA----CCAUugUAAUG- -5' |
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12305 | 5' | -47.7 | NC_003313.1 | + | 32856 | 0.68 | 0.849869 |
Target: 5'- uGguUUGGUGCUGC-GGUGGC--UGCa -3' miRNA: 3'- gCguAACUACGGCGaCCAUUGuaAUG- -5' |
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12305 | 5' | -47.7 | NC_003313.1 | + | 8816 | 0.68 | 0.840296 |
Target: 5'- gCGCA-UGAUGUCGCUcgaugcagaaaGGUGGguUUGCu -3' miRNA: 3'- -GCGUaACUACGGCGA-----------CCAUUguAAUG- -5' |
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12305 | 5' | -47.7 | NC_003313.1 | + | 32360 | 1.12 | 0.00174 |
Target: 5'- cCGCAUUGAUGCCGCUGGUAACAUUACa -3' miRNA: 3'- -GCGUAACUACGGCGACCAUUGUAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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