Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12306 | 3' | -58.4 | NC_003313.1 | + | 24937 | 0.66 | 0.416093 |
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Target: 5'- cCGCGauaAUCCAUgugauuggguuuGCCCACaGcGCGGCGUu -3' miRNA: 3'- aGCGU---UGGGUA------------UGGGUG-C-CGCCGCAc -5' |
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| 12306 | 3' | -58.4 | NC_003313.1 | + | 9099 | 0.66 | 0.397281 |
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Target: 5'- -gGCAGCgcaCCAUcaAUUCAUGGCGGCGa- -3' miRNA: 3'- agCGUUG---GGUA--UGGGUGCCGCCGCac -5' |
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| 12306 | 3' | -58.4 | NC_003313.1 | + | 22145 | 0.66 | 0.388083 |
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Target: 5'- gUCGUuuaGACCCGUacaccuGCCCAucCGGUuuagugaccaacGGCGUGg -3' miRNA: 3'- -AGCG---UUGGGUA------UGGGU--GCCG------------CCGCAC- -5' |
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| 12306 | 3' | -58.4 | NC_003313.1 | + | 12751 | 0.68 | 0.319692 |
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Target: 5'- aUGCcGCCCAUGCCCGCugGGaaGCGa- -3' miRNA: 3'- aGCGuUGGGUAUGGGUG--CCgcCGCac -5' |
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| 12306 | 3' | -58.4 | NC_003313.1 | + | 29049 | 0.68 | 0.311807 |
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Target: 5'- gCGCGGgUCAUuugUCgGCGGCGGCGUa -3' miRNA: 3'- aGCGUUgGGUAu--GGgUGCCGCCGCAc -5' |
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| 12306 | 3' | -58.4 | NC_003313.1 | + | 22064 | 0.71 | 0.18418 |
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Target: 5'- aUCGCcccGCCCGUGauaaggcugaUCACGGCGGCGa- -3' miRNA: 3'- -AGCGu--UGGGUAUg---------GGUGCCGCCGCac -5' |
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| 12306 | 3' | -58.4 | NC_003313.1 | + | 18337 | 0.73 | 0.143478 |
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Target: 5'- aUUGCAACCUcgccaGCCgagCGCGGCGGCGUa -3' miRNA: 3'- -AGCGUUGGGua---UGG---GUGCCGCCGCAc -5' |
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| 12306 | 3' | -58.4 | NC_003313.1 | + | 32763 | 1.09 | 0.000271 |
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Target: 5'- cUCGCAACCCAUACCCACGGCGGCGUGa -3' miRNA: 3'- -AGCGUUGGGUAUGGGUGCCGCCGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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