Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12306 | 5' | -55.4 | NC_003313.1 | + | 7295 | 0.71 | 0.26351 |
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Target: 5'- uUGCAGUUAUCaCAcuGCACCAAACCGAu -3' miRNA: 3'- -ACGUCGGUGGcGU--CGUGGUUUGGUUc -5' |
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| 12306 | 5' | -55.4 | NC_003313.1 | + | 11276 | 0.71 | 0.26351 |
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Target: 5'- uUGCGGCgcaaaGCCGCAGCGCauAGCgGAGa -3' miRNA: 3'- -ACGUCGg----UGGCGUCGUGguUUGgUUC- -5' |
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| 12306 | 5' | -55.4 | NC_003313.1 | + | 9910 | 0.71 | 0.26351 |
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Target: 5'- aUGUAGCCGCUGUgcuGCGCUcuGCCGAu -3' miRNA: 3'- -ACGUCGGUGGCGu--CGUGGuuUGGUUc -5' |
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| 12306 | 5' | -55.4 | NC_003313.1 | + | 21291 | 0.76 | 0.1119 |
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Target: 5'- gGCAGCUAUCGCGGgAUCAAACCu-- -3' miRNA: 3'- aCGUCGGUGGCGUCgUGGUUUGGuuc -5' |
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| 12306 | 5' | -55.4 | NC_003313.1 | + | 12493 | 0.76 | 0.10862 |
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Target: 5'- aGCGGCCACCGUgcucaguaauaaAGcCGCCAAuuuGCCGAGc -3' miRNA: 3'- aCGUCGGUGGCG------------UC-GUGGUU---UGGUUC- -5' |
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| 12306 | 5' | -55.4 | NC_003313.1 | + | 32800 | 1.08 | 0.000474 |
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Target: 5'- cUGCAGCCACCGCAGCACCAAACCAAGc -3' miRNA: 3'- -ACGUCGGUGGCGUCGUGGUUUGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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