miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12309 3' -48.1 NC_003323.1 + 3685 0.66 0.522411
Target:  5'- --gUCGGGGCCAUGgagGAGCCUUUUg -3'
miRNA:   3'- auaGGUUUCGGUGUaa-UUCGGAGAGg -5'
12309 3' -48.1 NC_003323.1 + 4473 0.67 0.484144
Target:  5'- -cUCCGAuuguguuGCCuCAUUAcuugcGGCCaUCUCCa -3'
miRNA:   3'- auAGGUUu------CGGuGUAAU-----UCGG-AGAGG- -5'
12309 3' -48.1 NC_003323.1 + 442 0.68 0.412013
Target:  5'- -cUCCcuGGCCGCuccccugGAGCuCUCUCUc -3'
miRNA:   3'- auAGGuuUCGGUGuaa----UUCG-GAGAGG- -5'
12309 3' -48.1 NC_003323.1 + 8658 0.68 0.412013
Target:  5'- -cUCCcuGGCCGCuccccugGAGCuCUCUCUc -3'
miRNA:   3'- auAGGuuUCGGUGuaa----UUCG-GAGAGG- -5'
12309 3' -48.1 NC_003323.1 + 1599 1.13 0.000205
Target:  5'- uUAUCCAAAGCCACAUUAAGCCUCUCCa -3'
miRNA:   3'- -AUAGGUUUCGGUGUAAUUCGGAGAGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.