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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
12310 | 5' | -55.7 | NC_003323.1 | + | 7152 | 0.66 | 0.210201 |
Target: 5'- aCCAcAGGaacaguUCCUGAgauugGAGCCUCCggGGCCa -3' miRNA: 3'- -GGU-UCC------AGGGUUaa---CUCGGGGG--UCGG- -5' |
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12310 | 5' | -55.7 | NC_003323.1 | + | 539 | 0.66 | 0.210201 |
Target: 5'- aCAAGGaaguagagaaagUCC----UGGGCCCCguGCCg -3' miRNA: 3'- gGUUCC------------AGGguuaACUCGGGGguCGG- -5' |
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12310 | 5' | -55.7 | NC_003323.1 | + | 8755 | 0.66 | 0.210201 |
Target: 5'- aCAAGGaaguagagaaagUCC----UGGGCCCCguGCCg -3' miRNA: 3'- gGUUCC------------AGGguuaACUCGGGGguCGG- -5' |
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12310 | 5' | -55.7 | NC_003323.1 | + | 733 | 0.68 | 0.140003 |
Target: 5'- -aGAGGugaucgaUCCCGGacGAGCCCCCA-CCu -3' miRNA: 3'- ggUUCC-------AGGGUUaaCUCGGGGGUcGG- -5' |
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12310 | 5' | -55.7 | NC_003323.1 | + | 6379 | 1.14 | 2.3e-05 |
Target: 5'- cCCAAGGUCCCAAUUGAGCCCCCAGCCg -3' miRNA: 3'- -GGUUCCAGGGUUAACUCGGGGGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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